| Aim: Chemotherapy is one of the most important cancer treatments today. The responses of different kind of cancer cells to the same chemotherapeutic drug are different, while the responses of the same cancer cell to different chemotherapeutic drugs are different as well. There is some relationship between some important genes expression and the sensitivity of the chemotherapeutic drugs to these cells. Researches have indicated that the targets of Stathmin gene product and two chemotherapeutic drugs (Vincristine and Paclitaxel) on cancer cells are located respectively on the same way (microtuble). The sensitivity of VCR and PTX to some cancer cells can be changed by decreasing the expression of Stathmin gene. By means of MTTcolorimetric assay, we selected eight different kinds of cancer cells to investigate the relationship between the expression of Stathmin gene and the sensitivity of the two chemotherapeutic drugs, which may contribute to a better drug-choosing for clinical cancer chemotherapy and may be helpful to further studies on the increase of the chemosensitivity of these two chemotherapeutic drugs.Method: (1) Eight kinds of cancer cell lines including Human Osteosarcoma cell(SOSP-9901 and SOSP-9607),laryngocarcinoma cell (Hep-2), gastric cancer cell (MKN45), cervical cacer cell(Hela), human lung cancer cell(A549), human hepatocelluar carcinoma cell (HHCC and 7721) were selected and cultured in PRMI1640 medium. Their growth status was monitored under inversion microscope. (2)Cells were transferred into 96 well plate and maintened in CO2 incubator for 24h. 50μL of each kinds of drugs in deffrent concentrations were added into different cell wells which were set up as treatment group(with drugs),control group(whithout drugs)and zero group. 48 hours later, 20uL MTT was added into each well for another 4h, and then 150μL DMSO was added. OD was valuated at the wave length of 490 nm, and inhibition rate was counted. (3) The eight kinds of cultruled cells (>106cells) were collected respectively and the total RNA of these cells were purified using Trizol method. After that, cDNA were synthesize and OD of cDNA was measured by protein nucleic acid analysis apparatus, then these different concentration cDNA were adjusted to the same concentration of 0.2g/L with ddH2O. UsingStathmin gene specific primer synthesized according to the sequence reported on Gene Bank, we examined the expression of Stathmin gene expression by Real- time RT- PCR in 20μL reaction component. To analysis the Fluorescence-cycle curves gained, Ct values showed the difference of the expression of Stathmin gene for eight kinds of cancer cells. Agarose gel electrophorasis of PCR products of Stathmin gene showed that there were specificity band at 450bp,which proved the specificity of PCR process.Results:(1) The morphology changes of these cancer cells in MTT assay showed that the majority of the cells (>95%) shrank 24 hours after being treated by drugs, and many of them (>50%) broke 48 hours later, while in the control group, the cells reproduced significantly in 48 hours, and covered 90% of the bottom of the well in 72 hours, (2) A549,Hep-2, 9901, 9607 were sensitive to both of two kind drugs. Among them, 9607 and 9901 cells were extremely sensitive to the two drugs, while Hela and A549 were moderately sensitive to the two drugs. HHCC cell was sensitive to VCR but not DOC. Hela cell was sensitive to DOC but not VCR. MKN45 and 7721 cells were sensitive to neither of these two kind of drugs. The dosage-effect curves showed that the inhibition rates of VCR and DOC to all of the cells increased with the rise of drugs' concentration. (3) The Ct values (the fluorescence-cycle curves of Stathmin gene PCR) of the eight kinds of cells were gained. The sequence of the Ct values from high to low was as follow: 7721, HHCC,MKN45, A549, Hep-2,...
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