Study On The Mechanism Of Haematotoxicity By Benzene And The Treatment Effect By Medicament

Objective: Study on the mechanism of haematotoxicity by benzene, and discuss the relation between mitochondrial DNA(mtDNA) 4834bp deletion in WBC and haematotoxicity by benzene. Study on the treatment effect by Rhodiola Crenulata (RC) on rats with benzene poisoning, and discuss pharmacological effects on the RC. Method: Adult female rats were given benzene of 0,0.4,0.8,1.6g/kg for 6 weeks (5d/w) ig. Blood cell counts, biochemical indexes, the main organic indexes (heart, liver, spleen, lung and kidney), the organic Pathology(liver, spleen and kidney), micronuclei of bone marrow cell, and the deletion of WBC mtDNA -4834bp were observed or tested. Before treatment rats with benzene poisoning, adult female rats were given benzene of 1.6g/kg for 3weeks (5d/w) ig.Rats with benzene poisoning were treat -ed by NS, 0.014g/kg batilol, 1g/kg RC, 2g/kg RC, 4g/kg RC. Blood cell counts, biochemical indexes, the main organic indexes (heart, liver, spleen, lung and kid -ney), bone marrow cell counts, ultrastructure of bone marrow cell, micronuclei of bone marrow cell, and serum SOD and MDA were observed or tested. Result: Experiment result show, the number of WBC,RBC,platelets and Hb level all decreased progressively in rats treated with benzene(P<0.05). As comparison with the test started, the number of WBC in the rats of 1.6g/kg group decreased to 50% at 1 week afer the test started. And the number of WBC in rats continuing decreased to 40% at the end of test. In the group of 1.6g/kg, at the end of the test, rats had intoxication symptoms such as increasing of ALT,CRE,LDH,ALP,the indexes of liver and kidney (P<0.05). In the group of 0.8 and 1.6g/kg, micronuclei of rats bone marrow cell decreased to 5.14-5.38‰from 2.5-2.6‰in normal rats(P<0.01). Ipofusc in spleen of rats treated with benzene increased obviously. There was no mtDNA-4834bp deletion in WBC of rats treated with benzene. Experiment result show, as comparison with model control group, the number of WBC were increased in the rats of 2g/kg RC group(P<0.05), and the granulocytic series were higher than model control group(P<0.01). In the group of 4g/kg RC, ALT,LDH(P<0.05) and micronuclei of bone marrow cell(P<0.01) were all higher than model control group. In the group of 1,2,4g/kg RC, the red cell series, and specific granules of myelocyte were higher than model control group(P<0.01). Conclusion: Animal model of haematotoxicity induced by benzene could be successfully duplicated with WBC decreased to 50% after treated with 1.6g/kg benzene(ig) 1 week. RC(2-4g/kg,5w) is effective in the treatment of WBC and bone marrow toxicity on rats with benzene poisoning.