Clinical Application Of FQ-PCR In Detecting Of HCV RNA In Serum And Peripheral Blood Monocular Cells

Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR)in detection of HCV RNA in serum and peripheral blood monocular cells(PBMC).Methods In 50 clinical serum specimens, HCV RNA was extracted from the serum and PBMC respectively, and detected by FQ-PCR, meanwhile the anti-HCV in the serum were examined by ELISA, the sensitivity and specificity were also compared between the two nethods.Results With the FQ-PCR, the positive rate of HCV RNA in the serum was 72% (3(?)(?)60) , among the 36 cases, 83. 3%(30/36) had a higher level of HCV RNA, which was more than 10~5 copies/mL, and the average level was 1. 2 X 106 copies/mL. The positive rate of HCV RNA in the PBMC was 60%(30/60) with an average level of 3. 1 X 104 cop-ies/mL. The level of HCV RNA in serum was obviously higher than that in the PBMC (t=4. 92,P<0. 01). With ELISA method, 34 cases were positive and the positive ratio was 68%, in which 30 were also HCV RNA positive. There were no significant difference between the two methods (x~2 = 0. l,P>0. 05) and the agreement was 80%. 3 cases are HCV RNA negtive in the serum while HCV RNA positive in the PBMC.Conclusions FQ-PCR is a simple and rapid method for detection of HCV RNA in serum and peripheral blood monocular cells, it may provide a reliable foundation for HCV clinical diagnosis and therapy effects monitoring, also the detection of HCV RNA in peripheral blood monocular cells is very important for further study on HCV.