Experimental Studies On Modified Polyhydroxybutyrate For Vascular Tissue Engineering

Tissue engineering is a new science of co-development and combination of modern cell biology, biomaterial science and engineering. The aim of tissue engineering is to investigate tissue and organ substitutes. Disease of blood vessel is one of the most common diseases. The best substitute of blood vessel has been pursued for a long time. Many materials have been tried such as autogenous artery or vein, artificial material, biomaterial et al? but the optimal substitute has not been found until now. Tissue engineering is a new and rapidly expanding field, in which a series of techniques are being developed for culturing and reconstructing a variety of tissues both in vitro and in vivo. Using those techniques, it is possible to reconstruct vascular model in vitro whose structure and function are as same as autogenous vessels.In this experiment the degradable material PHB was combined with another toughness material PEO, by the filature technic ofmake liquor dry, get a long and slender filament material, so the elasticity and toughness of the material improved. It is more suitable as scaffold for vascular tissue engineering. The material was embedded with collogen, then freeze-dried. Before seeded with rabbit VEC and VSMC, it was embedded with FN. A serial of experiments were used to assess the biocompatibility of the material with VEC and VSMC in vitro. Part IObjective: To provide cells resource for the experimental study on vascular tissue engineering. Methods: Vascular endothelial cells, smooth muscle cells were respectively isolated from rabbit aorta by enzyme digestion and tissue plant methods, subcultured, purified, identified and cells morphological observation, etc. Results: The morphological characteristics of two kinds of cells are accorded with those of vascular endothelial cells, smooth muscle cells. Conclusion: The cultured cells can meet the experimental expectations on vascular tissue engineering. Part IIObjective: Tubular porous vascular scaffolds and dynamicrota-tion culture technique were constructed. Methods: PHB was combined with PEO, by the filature technic of make liquor dry, get a long and slender filament material, PHB/PEO meshes were dipped into cross-linked typel collagen solution, dried under vacuum freeze condition in a special annular model. Results: The tubular porous scaffolds have elasticity, tenacity and hydrophilic, can maintain their shapes in culture medium for a long time.