| ObjectiveThe human retinal pigment epitheliumis ( HRPE ) cells' proliferation plays an important role on the development of proliferative vitreoretinopathy ( PVR) which its occurrence and development lead to the abortive cause of surgery of rhegmatogenous retinal detachment and it also is an occoecatio disease. The occurrence and development of proliferative vitreoretinopathy relate to the regulated informations which are out of control. Spikes coming from surrounding participate in signal transmission of RPE cells and activate the correlation factor of RPE cells, and precipitate RPE cells on the hypsi - vegetative state and prosoplasia. At present ,molecular bioresearch about proliferative vitreoretinopathy always is a hot topic to be studied by each nation's scholars.Protein kinase C is one of accommodative pathways for cells growth semaphore. The activated PKC mediates many varietas cell's biological effects by inducing Ser/Thr's residues of target protein molecules to generate phosphoryla-tion,and also is a signal regulatory enzyme about cellular biological signals'production and message's transmission and regulation, which impacts the metabolic conductive process of cellular bioinformation. Just for this reason, we chose whether RPE cells can generate changes of PKC activity and phenomenon of cellular proliferation or not as the study object to approach the mechanism which RPE cells'proliferation leads to PVR because of regulation system's changes of PKC signal, and the PKC inhibitor can hinder the process of RPE cells'proliferation.Material and Method1. The source of experimental material: The cultured RPE cells came from the eye ball which are after cornea transplantation in department of ophthalmology of the First Affiliated Hospital of China Medical University. The donor's is limited from 20 to 30. Collection of subretinal fluid and normal vitreous: All 24 patients with rhegmatogenous retinal detachment came from the department of ophthalmology of the First Affiliated Hospital of China Medical University and average age was limited between 43. 8 ±6 years old. Subretinal fluid was drawn- off from the eye being taken sclerotic cingulum operation, including B grade 12 and C grade 12. The collection of subretinal fluid was put and preserved in the sample tube below zero 70^. Subretinal fluid which was contaminated and was less than lml will be discarded. The normal vitreous came from donor' s eye ball.2. Culture and identification of human retinal pigment epitheliumis cells:To collect HRPE cells by trypsin.3. Groups of experiment: All of them were divided into four. Gowas blank, and positive control was Gj and G2. Experimental groups were G3B and G3C. G4 was the PKC specific inhibitor N, N - dimethyl. Time of being observed from zero to 24 hours.4. Determination of cell multiplication:To incorporate 3H -TdR to marker RPE cells'condition which synthesize DNA.5. Stimulating every group RPE cells and collecting PKC of cytoplasm and cytomembrance of RPE cells: To modulate RPE cells to final concentration in 105 ml ~ , every group RPE cells is respectively stimulated by subretinal fluid, normal vitreous, special activity activating agent PKC—PMA and culture solution. The extracts of PKC of cytoplasm and cytomembrance of RPE cells which were extracted from each group in preset time are preserved in the frige below zero 701 as enzyme preparation to be measured in the future.6. G4 was used to pretreat the RPE cells 20 minutes before the administration of SRF or PMA or normal vitreous, and then the activity of PKC was ob-...
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