The Effect Of BFGF On The Expression Of P53 And Apoptosis In Smoking Rat's Brain

ObjectiveBrain ischemia is a common pathologic state in neuro system. At present we have known much of the brain damage induced by acute ischemia, but the feature , mechanism , prevention and treatment of the damage induced by the chronic ischemia is still not clear. Apoptosis is a cell suicide duration which is characterized by initative, the interaction of some related genes, and DNA degradation in early period after the cell received certain kind of signal or suffered stimulate . It 's a way for cell death both under the physiologic and pathologic states , and it's relation with cerebravascular disease is more and more concerned of now. Smoking is an important variable risk factor for ICVD. Study showed that expose to the tobacoo smok can induce the apoptosis of cereberal cell, but the mechanism is not clear and the study of the prevention and cure for the apoptosis after smoking is rare. Our destination is to detect the effection of smoking on the expression of cerebral celll apoptosis and its accelerating factor P53, prove the relation between smoking and ischemic brain disease and the possible mechanism in it, study the effect of exogenous basic fibroblast growth factor (bFGF) on the expression of P53 and apoptosis in smoking rat's brain, and to find evidence for clinical prevention and cure by establishing a smoking rat model.MethodsEstablish a smoking rat model. 45 rats are divided into three groups: the normal group, the simple smoking group, andthe bFGF treated group at random. The terminal deoxynucleotidyl transfer-ase mediated dUTP-bootin lnick end labeling (TUNEL) technique and immu-nohistochemical method were used to detect the apoptosis and P53 protein expression. Use microimage analysis machine to collect the image and analysis the average gray seal of apoptosis cell and P53 positive cell in hippocampus. Use the SPSS11.5 statistic software for variance analysis and multiple comparison.ResultsThe expression of P53 and cell apoptosis are lower in the bFGF treated group than in the simple smoking group ( P <0. 01) ,and the expression of P53 and cell apoptosis in the simple smoking group and the bFGF treated group are higher than the normal group( P <0.01).First, P53 staining and expressionWe can see the P53 positive cells with blue nucleus and brown cytoplasm under the light microscope sight. They are distributed both in the cortex and hippocampus but more concentrated in the later part. There are a few positive cells in the normal group, and the expression of P53 possitive cell is higher in the simple smoking group and the bFGF treated group than in the normal one ( P < 0.01) ;and its expression in the bFGF treated group decreased dramatically than the simple smoking group (P <0.01). See the results in chart 1.Second, apoptosis cell staining and expressionThe positive staining of apoptosis cell under the light microscope is dark brown round or oval solid center body. They are mainly distributed in the hippocampus and few can be seen in the cortex. There are a few positive cells in the normal group, and its expression in the simple smoking group and the bFGF treated group is higher than in the normal one( P <0.01) ; in the bFGF treated group its expression decreased dramatically than the simple smoking group (P < 0.01). See the results in chart 2 .Conclusion1. Smoking can increase the cell apoptosis and P53 expression in the brain...