Effect Of Perfusion Into Peritoneum Cavity With Strong Acidification Electricity Water On Abdomen Adhesion

Strong acidification electricity water is a new sterilized reagent. Its sterilize capacity was physical and it was not irritated for human skin and mucosa. It was demonstrated that strong acidification electricity water was dephlogisticate for peritoneum inflammatory. In present study the histological characteristic of ileum sample from animal model and production of hydroxyproline was detected. The aim of study was to evaluation the prevention of strong acidification electricity water to abdomen adhesion.Material and methodsAnimal: 24 cases of health china rabbits, male or female. Body weighted from 2.0 to 2.5 kg Methods1. Preparetive of strong acidification electricity water: strong acidification electricity water was prepared with SUNTECH-1000 electrobath.Water which contain some salt was electrolyzed with equipment. Oxidation-deoxidation voltage of strong acidification electricity water was more than 1050 mv, and PH was less than 2.72. Animal experiment:Experiment group: 24 cases of china rabbit was divided into two groups randomly. Experimental group was treated with strong acidification electricity water. Control group was treated with nature solution. Every group contain 12 anmals.Anesthesia: animal was anaesthesia with 2% barbital benzene peritoniumly. Experiment procedure:Animal was anaesthesia and exploratory laparotomy with medioventral line incision. Membrana serosa of ileum was scraped with scalpel. Congestion maculation sized lcm×2cm formed which will resulted adhesion of intestinal wall. A plastic canula was detained in peritoneum cavity. 30ml per day of strong acidification electricity water(experimental group) or nature saline(control group) was perfused into peritoneum cavity through canula for five days consecutively after operation. Ten days after operation, the animal belly was opened and wound of ileum was observed.ObservationDegree of abdomen adhesion was measured with Philips criteria with naked eyes.Wound surface was observed with microscope, repair of wound was evaluated.Wounded ileum was harvested. Content of hydroxyproline was measured with Lowry method. 3. Statistical.All data was denoted as mean ±SD. Difference between groups was tested by X2 test. ResultsBody temperature surveillance: all animal survival normally within experimental period. Body temperature was measured through anus. Fluctuate of temperature was not more than 0.1Degree of adhesion: adhesion in control group was serious. It was different markedly with which in experimental group. Histological characteristic:With microscope, it was observed that wounded surface of serous membrane in experimental group was repaired entirely or partly. Little fibroblast was detected. In control group, most of wound was not repaired.Abundance fibroblast was detected. Inflammatory was serious. Measurement of hydroxyproline:Production of hydroxyproline in control group was 0.274±0.043, wheareas the production in experimental group was 0.201±0.018. The difference between two group was significant(P<0.05).ConclusionThe present study demonstrated that perfusion into peritoneum cavity with strong acidification electricity water can inhibited the production of hydroxyproline. As a result, the production of collage was inhibited. It was prophylaxis on abdomen adhesion.