Study On The Immune State Of Colonic Cancer And The Mechanisms Of Fas/FasL Mediated Immune Escape

Objective: colon cancer is one of the most commonly tumors of the digestive system in the world. In China the incidence of colon cancer is high, but the mechanism on the flare up of the colon cancer is not clear yet. [1]Recently,people become believe more and more the mechanism of immune escape of tumorous cells.The studied date demonstrated that the mechanism of Fas/FasL mediated immune escape is tightly related to occuring,development and clinical pathology of colon cancer. Fas is a member of tumor necrosis factor receptor (TNFR) superfamily, and FasL belongs to tumor necrosis factor (TNF) superfamily, Fas combine with FasL, and then induced apoptosisof target cell. Moreover, tumor cell throughf FasL of itself surface combines with Fas of activated T lymph cell surface, guide T cell to occur to die probably, escape the system of immunity watch. Abnormal expression of Fas system of human tumor cell made tumor organization can resist the immune watch and attack the immune system, then induced immune escape [2]. Cell immune is the most important way to fight tumor.Among these cell immune system , natual killer cell(NK),T lymph cell subgroup in tumor immune watch take the central role of adjusting control. Secondly, the red blood cells immunity which to be studied more and more in recent years plays an important role in fighting tumor mechanism. Abroad numerous research results show preoperated colon cancer patients, the active degree of NK cell was significantly lower than that of control group [3]. Colon cancer group CD₄⁺, CD₄⁺/CD₈⁺ is lower apparently than health compare group, and CD₈⁺ is higher apparently than comparing group [4]; The colon cancer patients garland rate of red blood cell notable below healthy crowd [5]. And along with Dukes stages increased, colon cancer patientss Immunity State is lower the more. And colon cancer patientss occur lymph node and far place to shift the more easily, prognosis is lacked the more. The immunity treatment of tumor has become a kind of relatively general means in recent years. A lot of researches show that the immunity function of tumor patients is low, we can improve immune function of tumor patients through the immunity conditioning agent of outside source, then reaches to fight tumor role. But clinical observation showed that the same immunity treatment scheme and its treatment effect have great difference. Therefore refer to its immunity state and the different individual of tumor patients, it is difference to reply for the immunity of tumor, and the immunity with tumor escape mechanism have very close connection. How to raise the effect of immunity treatment and reach the role that raises patient's immunity function really, it is the promising problem of the tumortreatment research in the future. In this study, we explored the relationship of the colon cancer patient's immunity state and tumor immunity escape. Colon cancer patients check the index of blood which reflects immunity function state, so we can know the tumor existence immunity escape phenomenon or not. Offer more valuable theory for the biological treatment of tumor. Methods: Immunohistochemistry analysis of Fas and FasL proteins was carried out in paraffin-embedded section from 30 surgically ressected colon cancers in the affiliated second hospital of HeBei Medical University. They were confirmed by pathology for colon or rectum gland cancer, that clinical information is complete. In which 18 male example and 12 female examples, age 32--72 years old, median age is 47 years old. Clinical pathology according to Dukes stages, 8 A issue examples, 10 B issue examples, 7 C issue examples and 5 D issue examples. Lymph node metastasis group (12) and non-lymph node metastasis group (18) were appraised by two pathology Directors. All patientss do not accept radio-treatment,chemical-treatment and hormone immunity treatment. Comparing with 10 group examples, for the normal colon mucous membrane, which is amputated. With immunohistochemistry analysis and the University of East China Immunohistochemistry analysis software .In the auto analysis optical microscope of 20 times, we check the express condition of Fas and FasL in colon cancer. The express quantityof Fas and FasL use the positive cell rate of area ratio (%). We compare colon cancer patients the Fas and FasL proteins express quantity in different Dukes stages and lymph node metastasis or not. The blood specimen is those above-mentioned patientss'vein blood before surgery. Adopting flow cytometry (FCM) check the quantity of the colon cancer patientss'T lymph cell subgroup and the C3b of red blood cell to get the quantity of the rate of body garland (E -C3bR). We compare colon cancer patientss'quantity of T lymph cell subgroup and C3b of red blood cell get body garland (E -C3bR) rate according to different Dukes stages and lymph node metastasis or not. Statistics handling use SPSS11.0 softwares. Lymph node metastasis group and non-lymph node metastasis group use T-test compared with two samples after the homogeneity of variance test; Different pathology stagess and compare group use one-way ANOVA; The positive cell rate of area ratio of Fas and FasL protein and each datum of immunity adopts linear correlation test. P < 0.01 is regard as having statistics meaning. Results: (1) according to Dukes stages, Fas and the FasL positive cell area ratio are significantly different in colon cancer A issue,B issue,C issue ,D issue and compare group. It has statistics meaning, P < 0.01. And the Fas positive cell rate of area ratio (%) decrease along with colon cancer stages progress reduce, and is below than that of health compare group; The FasL positive cell rate of area ratio (%) increase along with colon cancer stages progress increase, and is higher than that ofcompare group. (2) according to Dukes stages, colon cancer A issue,B issue,C issue,D issue and compare group,their T lymph cell subgroup and the C₃b of red blood cell quantity of the rate of body garland ( E -C₃bR ) are significantly different .It has statistics meaning, P < 0. 01. And CD₃⁺ ,CD₄⁺,CD₄⁺/CD₈⁺ and E -C₃bR (CD₃₅) reduce along with colon cancer Dukes stages progress, and below health compare group; CD₈⁺go up along with colon cancer Dukes stages progress, and is higher than compare grouping. (3)Colon cancer patients who have the transfer group of lymph node compared with who have not the transfer group of lymph node, their Fas,FasL,CD₃⁺,CD₄⁺,CD₄⁺/CD₈⁺,CD₈⁺ and CD₃₅ have statistics meaning, P < 0.01. The positive cell area ratio of FasL and blood level of CD₈⁺ in lymph node metastasis was higher than that of non-lymph node metastasis. Other index is lower . P<0.01. (4) The Fas positive cell rate of area ratio (%) is positively correlated with CD₃⁺,CD₄⁺,CD₄⁺/CD₈⁺and CD₃₅, and is negatively correlated with CD₈⁺; The FasL positive cell rate of area ratio (%) is negatively correlated with CD₃⁺,CD₄⁺,CD₄⁺/CD₈⁺ and CD₃₅, and is positively correlated with CD₈⁺; P < 0.01. Conclusion: (1) The positive cell rate of area ratio of Fas/FasL was remarkly different between lymph node metastasis and non-lymph node metastasis It has statistics meaning, P < 0.01. The positive cell rate of area ratio of FasL in lymph node metastasis were remarkly higher than that of non-lymph node metastasis, and Fas in lymph node metastasis were remarklylower than that of non-lymph node metastasis, according to Dukes stages, the Fas positive cell rate of area ratio (%) decrease along with colon cancer stages progress reduce, and is below than that of health compare group; The FasL positive cell rate of area ratio (%) increase along with colon cancer stages progress increase , and is higher than that of compare group. Refer to the Fas/FasL express level and colon cancer stages can be judged as a reference index of colon cancer clinical pathological stages. (2) According to Dukes stages, colon cancer A issue,B issue,C issue,D issue and compare group,their T lymph cell subgroup and the C₃b of red blood cell quantity of the rate of body garland ( E -C₃bR ) are significantly different .It has statistics meaning, P < 0. 01. And CD₃⁺ ,CD₄⁺,CD₄⁺/CD₈⁺ and E -C₃bR (CD₃₅) reduce along with colon cancer Dukes stages progress, and below health compare group; CD₈⁺ go up along with colon cancer Dukes stages progress, and is higher than compare group. Colon cancer patients who have the transfer group of lymph node compared with who have not the transfer group of lymph node, their Fas,FasL,CD₃⁺,CD₄⁺,CD₄⁺/CD₈⁺,CD₈⁺ and CD₃₅ have statistics meaning, P < 0.01. The positive cell area ratio of FasL and blood level of CD₈⁺ in lymph node metastasis was higher than that of non-lymph node metastasis. Other index is lower. P<0.01. Evidently, along with colon cancer Dukes by stages progress, the immunity function of patients is serious to damage, it makes tumor cell have more proliferation and metastasis. The immunity state of patients can...