| Among the nosocomial infections, fungi infection has become extremely serious. With the widely use of broad-spectrum antibiotics, anti-tumor drugs ,immunological inhibitors as well as the development of organ transplantation, catheterization and radiotherapy, especially the HIV infection which makes the patients with a low or defective immunological function, the infection caused by fungi that was thought to be conditional pathogenicity has become increased. At present fungi infection has contributed a major factor to the augmentation of incidence rate and mortality in the nosocomial infection. The research on drugs for shallow fungi infection has been gained some achievements, however the deep one is relatively lagged, which can not meet the clinical need. At present the anti-fungi drugs are mainly chemical ones, whose major problems are many side effects and toxicity. So the anti-fungi drugs with high-effect broad-spectrum, low toxicity has been a great interest for the pharmacologists. So seeking new active component for anti-fungi and the discovery of new target sites in the natural products has been become a hot spot.It has been reported that active component for anti-fungi are present in some herbs and plants, for example , garlicin has been applied to deep anti-fungi infection. Preliminary chemical analysis on ivy mosses showed that it contains saccharides,alkaloid, hydroxybenzene and flavone. To identify the anti-fungi active sites of ivy mosses, employed different extraction ways to extract dry ivy mosses, using the common clinical shallow and deep infectious fungi as a tested strain, this study conducted a in vitro anti-fungi experiment in different lixivium and abstracts of ivy mosses。This article discussed the influence of solvents and temperature on the anti-fungi result of ivy mosses and its possible mechanism .1 The experiments of ivy mosses ethanol extraction liquid against microorganism showed that broad-spectrum anti-microorganism components are present in ivy mosses.1.1 Ethanol lixivium has an inhibitory and killing effect on Asp. flavus,Asp. Niger, Penicillium with a MIC of 125μl/ml and a MBC of 250μl/ml, which provides a base for further studying the anti-pathogenic fungi activity of ivy mosses1.2 Ethanol lixivium had an inhibitory and killing effect on S.aureus,B.subtilis,P.aeruginosa, E.coli, Shigella dysenteriae bacilli,paratyphoid bacillus and P.vulgaris at different levels, with a MIC 31~125μl/ml and MBC 31~125μl/ml. Among those, coliform bacilli were more sensitive to ethanol lixivium, while B.subtilis,P.aeruginosa S.aureus were less sensitive to it.2 1:1 acetone solution was used to extract dry ivy mosses ,which lixivium was extracted by ethyl acetate。The extracted lixivium was double diluted by test tube for in vitro antifungal test. The observation showed that ethyl acetate extraction site existed broad-spectrum active antifungal components, to which shallow infectious fungi such as Microsporum canis and Epidermophyton flocclsum were more sensitive and deep infectious fungi such Candida albicans and Asp. Flavus were less sensitive.2.1 ethyl acetate lixivium A had an inhibitory and killing effect on Candida albicans, Cryptococcus neoformans,Asp. fumigatus, Scopulariopsis brevicaulis ,Microsporum grpseum and Microsporum canis at different levels with a antifungal concentration ranged from(15 ~125μl/ml )and the MIC and MBC values are the same.2.2 ethyl acetate lixivium B had an inhibitory and killing effect on Candida albicans, Cryptococcus neoformans,Asp. fumigatus, Asp. flavus, Asp. niger , Scopulariopsis brevicaulis, Scopulariopsis brevicaulis, Microsporum grpseum, Microsporum canis,Trichophyton mentagrophytes, and Epidermophyton flocclsum at different levels with a antifungal concentration ranged from( 7.5 ~ 125μl/ml) and the MIC and MBC values are the same.2.3 The extract, which was made by vaporize ethyl acetate lixivium B under depression was used to a in vitro antifungal test for the above fungi. Our results showed the lowest inh... |
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