Effects Of Alopering Alkaloid On Injured Liver Model And Immunity Function In Mice

Objectives: To investigate the effecfs of Alopering Alkaloid(AL) on injured liver model and immunity function.Methods: ig with AL (3mg/kg, 6mg/kg, 12mg/kg) in mice for 7 days, Three models was built by ip CCl4, GalN,iv BCG and LPS in mice Separately. The levels of ALT and AST in serum was examined and liver pothological injury was observed respectively. Spectrophotometry was used to determine LgM in mice, MTT colorimetric assay was used to determine the effect of AL on proliferation of B and T lymphocyte. The test in vitro with 3H-TdR incorporation was performed to explore phogocytosis of PMΦ and active of IL-2. In addition, the producing of TNF- a in mice was tested by ELISA.Result: 1.The rising of ALT, AST in serum induced by CCl4 was decreased significantly by AL( 12mg/kg); Compared with model, The Liver pathological injury of Three AL groups was obviously improved.2. The rising of serum ALT, AST induced by GalN was decreased significantly by AL(6mg/kg ,12mg/kg). The liver pathological observation showed that the liver injury of three AL groups was less than that of model to some degree.3.The rising of ALT, AST in serum induced by BCG and LPS was magnificently reduced by AL (12mg/kg). Compared with model, the liver injuries of three AL groups were alleviated to some degree.4. HC50 of three AL groups in mice was significantly reduced.5.The proliferation of B-lymphocyte was inhibited by AL in a concentration of 62.5-250 μg/ml.6.The proliferation of T-lymphocyte was promoted by AL in a concentration of 62.5-250 μ g/ml.7.The phagocytosis of mice PMΦ was inhibited by AL in a range of 62.5-250μg/ml.8.The production of TNF- a in mice was decreased by AL in a range of 62.5-250 μ g/ml.9.The active of IL-2 was promoted by AL in a range of 62.5-250μ g /ml.Conclusion: The liver injury induced by ip CCl4 in mice can be obviously improved by ig AL; The liver injuries induced by ip GalN or iv BCG and LPS were alleviated by ig AL to some degree.The producing of mice IgM,the proliferation of B-lymphocyte, the phagocytosis of PMΦ and producing of TNF- a in mice all were inhibited by AL; The proliferation of T-lymphocyte and active of IL-2 were promoted by AL. The results indicated that cell-mediated immunity can be promoted by AL, meanwhile, humoral immunity and nonspecific immunity function were inhibited.