The Role Of RANTES Expressed By Keratinocytes And Peripheral Blood From Patients With Psoriasis

Objective A viewpoint was proved by experiment,T lymphocyte played an important role in the pathogenesis of psoriasis,As a result,RANTES was gradually attented as a chemotactic factor that it could induce focal accumulation of T lymphocytes in psoriatic lesions.This experiment mensurated RANTES level in keratinocytes and peripheral blood from psoriatic lesions,another side,we tried to show differences in three stages of psoriatic patients for explained that RANTES induce T lymphocyte to walk out blood vessel and to accumulate in psoriatic lesions.Method The first part of the experiment,we mensurated RANTES level in keratinocytes from psoriatic lesions.Eight patients with psoriasis were diagnosed by clinic and pathology,included 4 male and 4 female,5 guttata type and 3 plaque,age from sixteen to sixty-eight, the course of diseases were from thirty days to six years. These patients had not been treated for half of year.Control group included eight healthy people,sex and age were not difference from test group.Chemotaxis of psoriatic lesional keratinocytes was detected by micropore loculus test. The concentration of RANTES was determined by ELISA in cultured supernatants of psoriatic lesional keratinocytes. In the second part of the experiment,we mensurated RANTES level in peripheral blood. Test group included eighteen psoriatic patients whose are diagnosed by clinic and pathology and they were in different stages of psoriasis.Age was from twenty-seven to seventy-six, course of diseases were sixty days to twenty years. Eight healthy peoples were selected in control group. RANTES level in peripheral blood was determined by ELISA.Results The chemotaxis of psoriatic lesional keratinocytes to T lymphocytes was stronger than that of controls (CI 2.955?.42>1.5?.26),P<0.05. The concentration of RANTES in the cultured supernatants from psoriatic lesionalkeratinocytes was also stronger than that of controls( 148?15.9pg/mL> 16.11 ?.2ng/m L), P<0.05. The results showed that RANTES level obvious increased in keratinocytes from psoriatic lesions.In addition,RANTES level in peripheral blood from patients with psoriasis also increased (55.22?1.92ng/mL>16.11?.2ng/mL), P<0.05.Meanwhile,we found that RANTES level in peripheral blood was not different in different stages of psoriasis ,perhaps,it just was implied no significant.Conclusions RANTES is chemotactic factor to memory T cells and activated naive T cells.Production of RANTES were induced by proinflammatory cytokines, in particular,TNF-a and IFN-y in combination showed marked enhancement of RANTES production in human synovial fibroblasts and endothelial cells. Therefore, psoriatic keratinocytes could produce RANTES because there were TNF-a and IFN-y here. Psoriasis vulgaris was a chronic cutaneous inflammatory disease characterized by epidermal hyperproliferation and defective terminal differentiation, and focal accumulation of neutrophils and T lymphocyte in the epidermis,it was unknown etiology by far.Increased levels of RANTES may be the cause of migration of the activated T cells to the epidermis of the psoriatic lesions. These results suggested that RANTES maybe play an important role in the inflammatory process of psoriasis. Our results further demonstrated keratinocytes regulatory effect in the infammatory process of psoriasis,It was significant that T lymphocyte can release some cytokine to impact keratinocyte and inflammatory cell in psoriasis.Our results showed that RANTES level did not chang along with the development of disease.