The Experimental Study Of CD44s, CD2/CD58 On Blood Cells And Serum Hyaluronan In The Patients With Psoriasis

There are neutrophils, lymphocytes and monocytes in the lesions of psoriasis and it basically demonstrate that psoriasis is an immune inflammatory disease. Many investigations indicate that adhesion molecules play an important role in inflammation, but the less had been researched about inflammation of psoriasis. DeGrendele et al found binding CD44s to hyaluronan (HA) mediates an extravasation at sites of inflammation and CD44s participating in CD2/CD58 (LFA-3) interactions can activate T lymphocytes. Yangxueqing et al found overexpression of CD44s was observed in basal cells and echinocytes through investigating adhesion molecule CD44 in the lesions of psoriasis. HA, a component of the extracellular matrix (ECM), is a major ligand of CD44s, which can inhibit prostaglandin E2 and oxygen-derived free radidicals releasing, as well as inhibiting rolling of inflammatory cells. But HA dependant binding CD44s also can cause aggregation of macrophages, lymphocytes and fibroblasts. This subject will show what adhesion molecules CD44s, CD2/CD58 play in inflammation of psoriasis from inflammatory cells rolling, activity of inflammatory cells and comparison with SLE.Part I The expression of CD44s on peripheral blood cells and serum HA in the patients with psoriasisObjective To investigate the effects of CD44s on peripheral blood cellsand serum HA in the patients with psoriasisMethods The expression of CD44s and HA wre studied by Flow cytometer(FCM) and ELISA.Results The HA of patients with psoriasis is higher than that in thenormal (P<0. 05). Lymphocyte, neutrophil CD44s and HA of active patientswith psoriasis are higher than those in the normal and stable patients(P<0. 05).Conclusion The CD44s and HA potentially participated in immuneinflammation of psoriasis. Overexpression of CD44s in active patients withpsoriasis can induce inflammatory cells extravasation into lesions ofpsoriasis and high concentration HA maybe inhibit the development ofinflammation.Part II The relation of CD44s,CD2 and CD58 on peripheral blood cells in thepatients with psoriasisObjective To investigate the relation of CD44s, CD2 and CD58 in the pathogenesis of psoriasis.Methods The expression of CD44s, CD2 and CD58 were studied by FCM. Results Lymphocyte and neutrophil CD44s in active patients with psoriasis are higher than those in the normal and stable patients with psoriasis (/><0. 05). The neutrophil CD2 of patients with psoriasis (active and stable ) is significantly lower than that in the normal (P<0. 05). The lymphocyte CD2 and CD58, lymphocyte CD2 and CD44s, neutrophil CD2 and CD58, neutrophil CD2 and CD44s, erythrocyte CD58 and CD44s are significantly positively correlated in the normal (PQ. 05).Conclusion The changed expressions of CD44s and relations of CD44s, CD2 and CD58 in active patients with psoriasis possibly participate in T cells activation and ignite outbreak of inflammation of psoriasis because of the broken immune balance. Part ITJ The comparison of CD44s, CD2/CD58 and HA between psoriasis andSLE Objective To investigate the variety of CD44s, CD2/CD58 and HA between-7-psoriasis and SLE.Methods The expression of CD44s, CD2 and CD58 and HA were studied byFCM and ELISA.Results HA of patients with SLE is higher than that in the normal andthe patients with psoriasis (P<0.05). Lymphocyte, neutrophil anderythrocyte CD44s in patients with SLE are lower than those in the normaland stable patients with psoriasis (P<0. 05), but lymphocyte CD58 is lowerthan that in the normal (P<0. 05) .Conclusion Many factors can affect HA of patients with psoriasis, soto detect whether psoriasis activates by HA needs more investigations.The difference of CD44s betwwen psoriasis and SLE indicates thatinflammatory cells possibly play different roles in two kinds disease.Compareing with the normal,...