Study On Separation Of Effective Site Clustes From Xiaoyu Tablet, Their Interactions Of Blood-lipid Regulation, And Mechanisms Of Increasing Stability Of Atherosclerotic Plaque

Objective: To investigate interactions of effective :;ite clusters separated from xiaoyu tablet on blood lipid-regulation, and observe protection of the site clusters on vulnerable atherosclerotic plaque and study its possible mechanis ns.Methods: Each site of Danshen and Shanzha exfacts was separated by polyamide-column chromatography and solvent extraction, respectively. The interactions of effective site clusters on blood lipid of hyperlipidemic trice were observed by orthogonal experimental design. A rabbit model of unstable atherosclerotic plaque which could be evoked into rupture was created. Effect of xiaoyu tablet ,consisted of effective site clusters ,on vulnerable atherosclerotic plaque was observed. Area of thrombosis on atherosclerotic aorta, morphologic character of plaque rupture, levels of blood lipid and lipoprotein, platelet aggregation and indexes of hemorheology, contents of 6-keto-PGF1α and TXB2 and their ratio in aorta, expressions of Bax protein and MMP-2 mRNA in aorta were determined by image analysis , electron and light microscope, chromatometry, turbidimetry, radioimmunoassay, immunohistochemistry, hybridization in situ, respectively.Results: (1) 75% ethanol site and ammonia site were separated from Danshen total phenol acid extract, Shanzha petroleum ether site,Shanzha acetone site and Shanzha anhydrous alcohol site were isolated from Shanzha total triterpenic acid extract (2) Danshen extract and Shanzha acetone extract were able to decrease the levels of serum TC and LDL-C synergically. Danshen extract and Shanzha absolute alcohol extract were able to increase the level of serum HDL-C synergically. (3)Xiaoyu tablet obviously inhibited thrombosis on atherosclerotic plaque and foam cell formation and aggregation in plaque, kept integrity of fiber cap and shoulder area. (4) Xiaoy a tablet down-regulated serum TC,TG and LDL-C in atherosclerotic rabbits, and reduced cholesterol content in abdominal aorta. (5)Xiaoyu tablet inhibited platelet aggregati 3n, especially at dose of 169 mg/kg. It increased the ratio of 6-keto-PGF1α/TXB2 in aorta of atherosclerotic rabbits. Xiaoyu tablet reduced blood viscidity , plasm viscidity, AIRC in atherosclerotic rabbits. (6) Xiaoyu tablet decreased positive cell expressions of Bax protein and MMP-2 mRNA inatherosclerotic aorta.Conclusions: Effective site clusters separated from xiaoyu tablet were able to regulate the levels of blood lipid synergically, and increase stability of atherosclerotic plaque of abdominal aorta in rabbits as well. The latter effect was associated with regulation of blood lipid , 6-keto-PGFi ?,TXB2 and their ratio in aorta, inhibition of platelet aggregation, reduction of blood viscidity and plasm viscidity, arid inhibition of Bax protein andMMP-2 mRNA expressions.