Proapoptotic Effect Of Angiotensin Ⅱ On Cultured Endothelial Cell And Protective Effect Of Xuezhikang

[Objective] To clarify whether AngⅡ induce apoptosis of cultured HUVECs,and to investigate the protective effect of Xuezhikang. To elucidate the proatherogenic effect of AngⅡ and the non-lipid mechanisms of Xuezhikang.[Methods]Cultured ECV304 cells were randomly divided into ①,②and③ groups. Group ①: control ; Group ②: Cells were stimulated with AngⅡ(10-8,10-7,10-6,10-5,10-4mol/L)for 18h. Group ③: Cells were exposed to AngⅡ(10-4mol/L) and interfered with Xuezhikang(500ng/ml) for 18h.The growth inhibition of cells induced by AngⅡ(10-8,10-7,10-6,10-5,10-4mol/L)was assessed by ATP-biolum inesence Assay. The change of apoptosis of HUVECs was investigated by electron microscope. The activity of Caspase-3 was assayed by spectrophotometry . Flow cytometry was used to measure the apoptosis ratio induced by AngⅡin different concentractions ,and to determine the effects of Xuezhikang . [Results]①Compared with controls , HUVECs were inhibited by different concentractions (10-8～10-4mol/L)of AngⅡ(P<0.05). These alteration was in a dose-dependent manner. ②Apoptosis ratio was induced by AngⅡ(10-7～10-4mol/L)with increase of concentrations (P<0.05).The activity of Caspase-3 was significantly higher in the AngⅡ(10-4mol/L)treatment group than in the control group(P<0.01). ③ The change of apoptosis of HUVECs may be observed by electron microscope. ④A desease in apoptosis cells were observed when Xuezhikang(500ng/ml)and AngⅡ(10-4mol/L) used jointly. [Conclusions]Apoptosis may be induced by AngⅡand the ratio increases with the rise of concentrations, and Xuezhikang may reduce apoptosis ratio of endothelial cells, which might be helpful to explore the pathogenesis of atherosclerosis and the non-lipid mechanisms of Xuezhikang.