| Objective:Xinshu oral liquid is extracted and prepared from Radix Angelicae sinensis, Rhizoma Chuanxiong and Flos Carthami. It is commonly used for the treatment of coronary heart disease, angina pectoris, arteriosclerosis and high cholesterol. In this thesis, Xinshu oral liquid was studied by both serum pharmacology and serum pharmacochemistry, and a preliminary attempt for combination of serum pharmacology and serum pharmacochemistry was explored.Methods and Results:1. The serums containing Xinshu oral liquid were attained at different time points from the rabbits given by Xinshu oral liquid. The serums were separated instantly. Passaged vascular smooth muscle cells (VSMC) were cultured in vitro with the serum containing Xinshu oral liquid, and the growth of the cells was observed by dying with MTT. The results showed that the proliferation of VSMC was significantly suppressed by positive control and the serum contain Xinshu oral liquid which were attained at 0.5h~1.5h in the 4g/ml dose group and which were gained at 1.5h in the 2g/ml dose group compared with blank control (p<0.05, p<0.01).The inhibiting effects on the proliferation of the cells were significantly higher in the group of the serum containing Xinshu oral liquid attained at 1.5h in the 4g/ml dose group than in the group of positive control (P<0.05). 2. The serums containing Xinshu oral liquid were attained at different time points from the rabbits given by Xinshu oral liquid to investigate the prohibitive effects of the blood platelet aggregation rate in vitro caused by both arachidonic acid (AA) and adenosine diphosphate (ADP). The results showed that the blood platelet aggregation rate in vitro caused by AA and ADP were all significantly suppressed by positive control and the serum containing Xinshu oral liquid which were attained at 0.5h~6.0h in both 4g/ml dose and 2g/ml dose groups and which were gained at 0.5~4.0h in the 1g/ml dose group, compared with blank control (p<0.01). In addition, the inhibiting effects on the platelet aggregation rate of the groups which dose was 4g/ml that were attained at 0.5~1.5h and which dose was 2g/ml and 1g/ml that gained at 1.0h were higher than those on the group of positive control (P<0.05, p<0.01). 3. HPLC methods for the determination of serum concentration of ferulic acid (FA) were developed, and there was no interference from endogeneous substance in serum. The linear range for ferulic acid was 0.1~0.8μg/ml, the minimum quantitation limit was 50ng/ml. The within-day precision (RSD) were 3.98%, 4.36% and 3.26%, the between-day precision (RSD) were 4.21%, 5.62%, 3.51%, and the recovery rate were 104.71%, 88.29%, 87.75%, respectively. All above were in accord with the requirements of clinical biosamples assay.4. HPLC methods for the determination of serum concentration of tetramethy pyrazine (TMP) were developed, and there was no interference from endogeneous substance in serum. The linear range for TMP was 0.05~0.4μg/ml, the minimum quantitation limit was 25ng/ml. The within-day precision (RSD) were 6.73%, 3.38% and 3.26%, the between-day precision (RSD) were 6.50%, 5.35%, 3.39%, and the recovery rate were 82.92%, 84.41%, 86.87%, respectively. All above were in accord with the requirements of clinical biosamples assay. 5. The relationships of the determined concentrations of both FA and TMP which were independent varies and the suppressive rate of the proliferation of VSMC and the aggregation of blood platelet which were dependent varies were studied with the statistic method by SPSS software. A simple correlation test and multiple linear regression were implemented to analyze the relationships. The results showed that the determined concentrations of FA and TMP had relationships with both the suppressive rate of the proliferation of VSMC and that of the aggregation of platelet caused by AA (p<0.05,p<0.01). Co-operative effects caused by FA and TMP (R2=0.450) were better than the individual effect produced by either FA (R2=0.365) or TMP (R2=0.264). So, Y1=107.339X1+58.014X2-9.555 was... |
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