Researches Of Plasma Lysophosphatidic Acid And Phosphatidic Acid Concentration In Experimental Rats With Cerebral Thrombosis

Objective To research the value of the dynamic changes of plasma lysophosphatidic acid(LPA)and phosphatidic acid(PA) concentration in rats with cerebral thrombosis. Methods Cerebral thrombosis rats model were produced through injecting thrombin into the internal carotid artery (ICA) by introducing a catheter. Observe the changes of plasma LPA and PA dynamically. The degree of the impairment of neural function was scored and the histological changes were evaluated using light microscopy.Results The results showed that the concentration of plasma LPA and PA in control group were low(2.41±0.17μmol/L, 3.77±0.26μmol/L respectively) and both of them increased in rats with cerebral thrombosis. The concentration of plasma LPA and PA in rats of the 2nd experiment group was significantly higher than other 5 groups(P﹤0.05). The concentration of plasma LPA decreased rapidly and 6h after the surgery they had no significant differences compared with control group(P>0.05).By contrast, the concentration of plasma PA decreased slowly and 12h after the surgery they still had significant differences compared with control group(P﹤0.01).There was a significant positive correlation between the concentration of plasma PA and the impairment of neural function(r=0.947,P﹤0.01).Conclusions In experimental rats with cerebral thrombosis, the rapidly increasing concentration of plasma LPA suggests that LPA maybe an important marker to help diagnose hyperacute cerebral infarction. The changes of plasma PA concentration between 4 experiment groups and control group suggest that PA could reflect the impairment of neural function. The relation between PA and the impairment of neural function maybe indicate that PA could reflect the degree of cerebral lesion as a "marker of cerebral ischemia ".