Correlation Studies Of MSCT Perfusion Imaging With Tumor Angiogenesis In Hepatocellular Carcinoma Of SD Rats

Objective : To investigate the correlation of perfusion parameters with vascular endothelial growth factor (VEGF) protein expression and microvessel density (MVD) in hepatocellular carcinoma of SD rats.Materials and Methods : Walker-256 Cancerous ascite was injected into rat liver directly after concentrated by centrifugal effect to establish hepatic cancer model in SD rat; 10 normal rats and 20 rats withWalker-256 hepatic cancer undergone single slice dynamic CT in liver by using Bodyperf series after an intravenous bolus contrast material injection ,Using DynEva soft to generate time density curve (TDC), The perfusion parameters were calculated by using maximum slope method and compared; Immunohistochemical stain methods were used to demonstrate the situation of VEGF protein expression and quantitatively measure the MVD in liver tumor of rats. Spearman rho correlation analysis was used for observing the correlation VEGF and MVD with the perfusion parameters.Results: Ratio of tumor raise was 100% in rats. There were statistical significant in CT perfusion parameters between hepatocellular carcinoma and normal liver in rats. Spearman correlation analysis demonstrated that were no correlated with both MVD and VEGF (r=0.415,p=0.087) ,CT perfusion parameters were no correlated with MVD and VEGF (P>0.05) .Conclusions: The changes of some perfusion parameters of dynamic enhance CT may be useful in the quantitative estimation effects of hepatic carcinoma in rats, but they are not correlated with MVD and VEGF protein expression .