The Clinical Study On The Changes Of Serum, Urinary Col-â…£,LN Of Chronic Nephropathy And The Relationship With Zheng Differentiation-Classification

Objective:1.To study the relationship between the level changes of serum, urinary Collagen IV(Col-IV) > Laminin(LN)and endogenous creatinine clearance, and the application value in Chronic nephropathy patients; 2. To study the relationship between different indexes and Zheng differentiation-classification of TCM (liver yin and kidney yin deficiency, qi-yin asthenia, spleen yang and kidney yang deficiency): 3.To study the relationship between different indexes and urine protein of 24 hours; 4. To evaluate the correlation between the serum Col-IV LN and the urinary Col-IV LN in Chronic nephropathy patients; 5. Which can reflect the lesion of the renal better in the serum Col-IV LN and the urinary Col-IV LN.Methods:The serum, urinary Col-IV> LN in 90 Chronic nephropathy patients and 20 healthy controls were measured respectively by two-layer antibody sandwich enzyme-linked immunosorbent assay(ELISA) method; Liberty urinary creatinine were measured by bitter acid method.Results:l.The contents of serum urinary Col-IV LN in 90 Chronic nephropathy patients increased significantly compared with the control group; 2. Compared with the control group and the renal failure phase group, the contentsof serum Col-IV LN in failure compensation phase group increased significantly, P<0.01; Compared with compensation phase group, P<0. 05;3. Compared with the control group and the renal failure phase group, the contents of urinary Col-IV LN in failure compensation phase group increased significantly, P<0. 01; Compared with compensation phase group, P<0.05;4. Compared with the control group the spleen yang and kidney yang deficiency group the contents of serunu urinary Col-IV LN in qi-yin asthenia group increased significantly, P<0. 01; 5. Compared with the control group the spleen yang and kidney yang deficiency group the contents of serum Col-IV in group liver yin and kidney yin deficiency group increased significantly, P<0.05; Serum LN and urinary Col-IV LN increased significantly, P<0. 01; 6. There was no correlation between the serum urinary Col-IV LN and the urine protein of 24 hours; 7. There was low correlation between the serum Col-IV and urinary Col-IV the serum LN and urinary LN.Conclusions: 1. With the lesion of the renal function , the contents of serum, urinary Col-IV LN increased significantly in Chronic nephropathy patients. Both Col-IV and LN took part in the pathological mechanism of the glomeruloselerosis: 2. Different indexes in the failure compensation phase group were the highest, urinary Col-IV LN can reflect the lesion of the glomerulus and the renal tubule interstitial; 3.In Zheng differentiation-classification of TCM , the contents of serunu urinary Col-IV LN were the highest in qi-yin asthenia group, and there was significant difference compared with the spleen yang and kidney yang Deficiency group; Liver yin and kidney yin Deficiency group was subsequent, there was significant difference compared with the spleen yang and kidney yang Deficiency group too. The difference in the levels of serum urinary Col-IV LN might be one of interal factors which formed different TCM syndrome types of Chronic nephropathy.