| Objective:To develop the compound group of epidermal growth factor (EGF) and hyaluronic acid (HA),to observe the mechanism and the effect of the compound group on the wound healing of rats.To provides an experimental basis for clinical application.Methods:1.Preparation of the compound group made from EGF and HA:Under the asepsis conditon,the compound group of EGF and HA was completed.In the same condition ,the other three groups, EGF group ,HA group and polyvinyl alcohol group were completed.2.Seventy-two male SD rats were randomly divided into four groups.Under general anesthesia ,two 5cm cuts were made on two sides,parallel to the spine with the deep from epidermis to the muscle layer.Then the cut was sewn with 3~0 suture. The cuts of four groups were applided respectively with compound group of EGF and HA, EGF group, HA group and polyvinyl alcohol group(control group),and then bound up with sterile gauze.On the 3rd,5th,7th,9th,14th and 21st days after the surgery,3 rats in each group were killed to be collected to specimens.The expression of EGF,HA in skin was detected with Streptavidin/Peroxidasa(SP) immunohistichemical methods. Under light microscope (LM),the histologic change was observed and the content of PCIII was determined.Result:1.General observation: After surgery ,in three treatment groups the wound were clean,with little seepage on the surface of the wound,slight inflammation,no obvious red and swollen around the cut,and the healing line was not clear.In the control group ,there were escharosis on the surface of the wound, much more seepage,the wound was hard to clean,red and swollen around the cut and there was an obvious healing line after the wound healing. 2.Observation under LM:After surgery ,compared with cotrol group,in compound group group,there was few inflammatory cell infiltrated on both sides of derma incision;the proliferation of acanthoid cell layer was not obvious;the FBs with a bit small body and slim nucleus spread loosely;quite few new collagen fibers could be seen on both sides of the cut,with comparably small bunches of loosely spread collagen,and they were slight keratinized.EGF group group and HA group group were superior to control group,inferior to group compound group group.3.Distribution of HA in the skin: the upper part of the epidermal and the dermis of compound group group showed HA stain strong positive on the 3rd,5th,7th day,and turned to positive on the 9th day;the below part of the epidermal turned from positive to weak positive. The epidermal of EGF group group showed HA stain strong positive on the 3rd day,and turned to negative on the 9th day,and the dermis turned from strong positive to positive.HA stain of HA group group was similar to compound group group.The below part of the epidermal of control group showed HA stain positive on the 7th,9th day,showed negative on the 3rd,5th day;the dermis showed HA stain weak positive on the 3rd day,turned to negative on the other days. 4.Distribution of EGF in the skin: the upper part of the epidermal of compound group group showed EGFstain strong positive on the 3rd,5th,7th ,9th day; the below part of the epidermal and fibroblast and hair follicle showed EGFstain positive on the 3rd,5th,7th day,and turned to weak positive on the 9th day.The upper part of the epidermal of EGF group group showed EGFstain strong positive, the below part of the epidermal and fibroblast and hair follicle showed EGFstain positive.The epidermal of HA group group showed EGFstain positive. The epidermal of control group showed EGFstain positive on the 3rd day, the hair follicle weak positive; fibroblast and hair follicle showed EGFstain positive on 5th,7th and 9th day.5.PCIII content: the content of compound group group was significant higher than control group on all point time (P<0.05);the content of PCIII in EGF group group and HA group group was obviously higher than control group on all days except the 21st day(P<0.05);the content of PCIII in compound group group was obviously higher than EGF group grou...
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