| Thyroid carcinoma is the most familiar thyroid malignant tumor, it consist of 1% in all malignant tumor. Differentiated thyroid carcinoma includes papillary thyroid carcinoma(PTC) and follicular thyroid carcinoma(FTC), both account for 80%~90% of total thyroid carcinomas. Given early surgical therapy, 5 years survival may higher to 75%, it illuminates the importance of early diagnose. With the deeper recognization of the thyroid carcinoma and development of diagnose technology, the occurrence rate of the thyroid neoplasm tend to ascend in recent years. Therefore, to discuss the carcinogenesis, progression and metastasis mechanism of thyroid carcinoma have an important basis and clinical significance.The carcinogenesis and progression of tumor are a very complex biological phenomenon with multi-factor, multi-gene, and multi-phases. It is well known that many signals transfer factors participate in this process, the discovery of relationship between some signal transfer factor and the carcinogenesis and progression of tumor make research more activity. Wnt signal transduction is an important approach to control the growth, progression and differentiation of cell, the mutation of APC and P-catenin promote this pathway aberrant activation, which make the concentration of dissociative P-catenin ascend and enter into nucleus, promote the transcription of target gene cyclinDl and c-myc, cause cell proliferation and occurrence of tumor, p-catenin is an important control point of Wnt signal transduction pathway. Otherwise, P-catenin is an important segment of E-cad/cat compound, and the abnormal of P-catenin make the structure and function of whole compound out of the way, and it makes an important role in the progression and metastasis of tumorUntil now, the mechanism of thyroid carcinoma's progression and metastasis has not been elucided. So investigating the expression of P-catenin, cyclinDl and c-mycprotein and the relationship between them may shed a light on discovering the mechanism of thyroid carcinoma's progression and metastasis, and it provide the basis of research to inhibit tumor metastasis and gene therapy. We investigate the expression of P-catenin, cyclinDl and c-myc in thyroid normal tissue, thyroid adenoma and DTC by SP immunohistochemical technique, to discuss its role in carcinogenesis and progression of thyroid carcinoma and the relationship between them.Materials and methodsWe have collected 95 specimens from surgical resections, and all the sections were formalin-fixed and paraffin-embedded, each has detailed clinical data, the HE staining sections were affirmed and histopathologic types were classified by two pathologist. Thyroid follicular adenoma 28 cases, 17 male and 21 female, aged between 15 to 80 years, average age is 42.89 15.27. DTC 57cases, 14 male and 43 female, aged between 14 to 70 years, average age is 41.91 15.11 years, 48 PTC and 8 FTC. All cases were not performed any radiotherapy and immunotherapy. According to the TNM classification of UICC, 41 patients had stage I tumor, 7 stage II , 9 stage III, 1 stage IV. Choose 10 normal thyroid tissues around thyroid adenoma as common comparison. P-catenin, cyclinDl and c-myc protein expression in 10 normal thyroid tissue, 28 thyroid adenoma and 57 DTC sections were detected by immunohistochemistry streptavidin peroxides methods. The results were analysis by SPSS 10.0 statistical software, a equal 0.05 were considered as significant test level.Result(1) p-catenin was normally expressed in all of normal thyroid tissue, while cyclinDl and c-myc were negative.(2) The aberrant rates of P-catenin expression in thyroid adenoma and DTC were 17.9% (5/28), 57.9% (33/57) respectively, there was significant difference (P< 0.05).(3) The positive rates of cyclinDl expression in thyroid adenoma and DTC were 35.7%(10/28), 64.9%(37/57) respectively, there was significant difference (P<0.05).(4) The positive rates of c-myc expression in thyroid adenoma and DTC were 25.0 %(7/28), 52.6% (30/57) respectively, there was significant...
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