Experimental Research On The Cellular Signal Transduction Mechanism Of The Detoxification Of Liangge Powder

Objective : To study on the cellular signal transduction mechanism of the detoxification of Liangge Powder.Methods: The experiment was composed of tow parts, in vitro and in vivo. In vivo, the animal model of LPS was produced. Some items were used to observe the effects. The levels of TNF- a and IL-6 in the lung homogenates were tested by using the ELISA. The change of NF- K B in nucleoprotein detected by EMSA. In vitro, normal rats were medicated with Liangge Powder and the serum was collected to prepare the medicated serum. The macrophage was incubated with different dose medicated serum. Then, the expression change of membrane CD14 (mCD14) stimulated by LPS in macrophage was detected by ISH. The change of p38MAPK were observed by radioactive isotope.Results: In vivo, the levels of TNF- a and IL-6 in lung homogenates after injected by LPS were risen, respectively, which were significantly higher then that of the blank-control group. The level of TNF- a was significantly lower at high-dose group then that of the other dose groups at 2h. Later, the different dose groups also decreased at 4h and 8h. Compared with the LPS model group, the different dose medicated groups decreased the level of IL-6 at 2h. The LPS model and low-dose group peaked at 4h and reduced at 8h. However, the high-dose group peaked at 2h and decreased gradually at 4h and 8h. The effect of removing toxin of Liangge Powder depend on the dose. The dose higher the levels of the two cytokines lower. Compared with the LPS model group, the different dose groups decreased the activity of NF- K B in the nucleoprotein of lung tissue. The high-group was significantly lower then that of other groups at 4h. In vitro, the expression of mCD14 were feeble in the RAW264.7, which stimulated by LPS for 1h and cultured with high-dose Liangge Power medicated serum. The mediate-dose and low-dose were distinctly higher then that of the high-dose. Cultured with normal rat serum, the expression was notable positive. The blank-control-4-group was negative, however. Compared with the LPS model group, the activity of pSSMAPK were lessened in RAW264.7 were incubated by different dose medicated serum and comparison medicament. The high Liangge Power medicated serum could inhibit the activity of p38MAPK. The activity was highest incubated by normal rat serum, however.Conclusions: Liangge Powder could retain the detoxification. The mechanism maybe: l.the function of lessening the levels of TNF- a and IL-6 .2. the function of restraining the activity of NF- k B. 3. the function of inhibit the expression of mCD14 in RAW264.7. 4. the function of control the access to MAPK.