Comparison Of The Outcome Of Bridging By Skeletal Muscle (Semi-anastomsois And Semi-implantation Method) And Nerve Graft For Defect Of The Peripheral Nerves: On Experimental Study

Peripheral nerve defect is very familiar to clinician. When peripheral nerve defect caused by traumatism or operation is too long to sew up directly, it needs a implantation to act as a bridge to show the never rebirth successfully. However, the most effective method is nerve graft with own. But it would cause some functional disorder of provider. Proved by experiments, metamorphic skeletal muscle can be used as an implant to renovate short peripheral nerve defect. In 2001,When Xiehongling found the traditional method by metamorphic skeletal muscle not only needed long-playing,but also operated hardly. So they selected some Wister rats, on experimental study,the end they used semi-anastomsois and semi-implantation with skeletal muscle repairing the damaged peripheral nerves succeeded .It proved that unmetamorphic skeletal muscle could act as the bridge for nerve rebirth under abundant blood .To evaluate the result to semi-anastomsois and semi-implantation with skeletal muscle and nerve graft after the peripheral nerves injury. We selected 15 Wister rats (weight of 200～300ｇ) to experiment.We sutured the sciatic nerve by semi-anastomsois and semi-implantation with skeletal muscle on left. Methods:"Ｔ"type incisions were made at both ends of the bridging muscle .The transverse incisions were vertical to the longitudinal axis is of bridging muscle,and the longitudinal incisions were paralle to it .The depths of longitudinal incisions should be enough to bury the distal and prosimale end of free nerve. Then,we brought both edges of the superficial half part of epineurium and epimysium into contact connected the mbyanas to mosis,finally,sewed up the two edges of epimysia from both longitudinal incisions. At the same time,right common peroneael nerve was treated by the nerve graft,changes of the sides were measured after three months.Results:The conducting speed of motor nerve was,respectively,27.66±4.98m/s,28.85±5.9m/s(p＞0.05). The incubation period was,respectively,2.3±0.1ms,2.1± 0.1ms (p＞0.05).The change of waves,respectively, 11.9±0.6mv,12.9±0.5mv(p＞0.05).A great number of nerve fibers and myelination were found in histological sections.The counts of modulated nerve fiber were,respectively,457.20±20.31,475±21.87,Ｐ>.05, No significant difference was made between the two groups in the number regenerated fiber cross sectional area. Conclusion: (1) Finally,the distal and proximal part of nerve end meat each other by anastomosis,and the function of denervated muscles was stored. (2) The effect of semi –anastomsois and semi –implantation with skeletal muscle was as same nerve graft.