| At modern time,tumor is one of major diseases that threat the health of people.Anti-tumor medicine is one of the effective therapeutic approaches for tumor theatment.Thus,it is urgent for people to search for effective and low-toxin anti-tumor medicine.Because some of organ-metallic polyoxome-talates are low toxic material and have anti-tumor effect that it becomes a hot spot of study to develop a polyoxometalate anti-tumor medicine.This study use (Me4N)4[(a-SbW9O33)2(GeCH2 CH2CO2H)3] (Orgenogermanium Polyoxometalate),which synthesized first by Professor.Jianxin Li who is belong to the Northeast Normal University to investigate the anti-tumor activety in vivo and the mechanism of anti-tumor activity.1．Toxicity of Orgenogermanium Polyoxometalate in Vitro The study used MTT method to detect the toxic activity of Orgenogermanium Polyoxometalate on FL cell lines and L929 cell lines.The concentration of Orgenogermanium Polyoxometalate study used was 1280μg/ml,640μg /ml,320μg /ml,160μg /ml,80μg /ml,40μg /ml,20μg /ml,10μg /ml,5μg /ml and 2.5μg /ml.The results showed that experimental concentration of Orgenogermanium Polyoxome- talate was no toxic activities on both cell lines(p>0.05).2．Anti-tumor Activity of Orgenogermanium Polyoxometalate in Vitro The study used MTT method to detect the anti-tumor activity of Orgenogermanium Polyoxometalate on six cancer cell lines: SMMC-7721,Hela,K562,SGC-7901, EL-4 and B16 cell lines.The results showed that Orgenogermanium Poly- oxometalate inhibitedthe growth and proliferation of cancer cells listed above in vitro on 1280μg/ml,640μg /ml,320μg /ml,160μg /ml,80μg /ml(p<0.05). Orgenogermanium Polyoxometalate inhibited the growth and proliferation of cancer cells include SMMC-7721,Hela,K562 and EL-4 on 5μg /ml(p<0.05).3．Effect Cell Cycle and Cell Apoptosis of Orgenogermanium Poly- oxometalate in Vitro The study used One-Fluorescence Dying method on Flow CPPometer to detect the cell cycle and apoptosis of Orgenogermanium Polyoxometalate on SMMC-7721.The concentration of Orgenogermanium Polyoxometalate study used was 320μg /ml and 5μg /ml.The results showed that experimental concentration of Orgenogermanium Polyoxometalate can delay G0/G1 and shorter S,G2+M of cell cycle(p<0.05).The experimental concentration of Orgenogermanium Polyoxometalate can not transform cell apoptosis.4．Anti-tumor Study of Orgenogermanium Polyoxometalate in Vivo The study set up C57 bearing H22 mice model,which were 18-20g and male mice.Then treated with Orgenogermanium Polyoxometalate (30mg/kg, 60 mg/kg,120mg/kg) for 10 days.After that,the study measured mouse weight,tumor weight,thymus weight and spleen weight.The result of mouse weight showed that there was no significantly difference between experimental groups with control group(p>0.05),there was significantly difference between CPP group with control group. The result of tumor weight showed that experimental groups and CPP group were obviously less than control group(p<0.05). The results of thymus weight and spleen weight showed that experimental groups were obviously more than control group and CPP group(p<0.05).5．T LymphoCPPe Transformation Study of Orgenogermanium Poly- oxometalate in Vivo The study used 3H-TdR method to detect the T lymphoCPPe transformation of Orgenogermanium Polyoxometalate on C57 bearing H22 mice. The results of T lymphoCPPe transformation showed that 60 mg/kg and 120mg/kg groups were more than control group(p<0.05).CPP group was less than control group(p<0.05). Experimental groups were more than CPP group(p<0.05).6．NK,LAK CPPotoxicity Study of Orgenogermanium Polyoxometalate in Vivo The study used 3H-TdR method to detect the NK,LAK CPPotoxicity of Orgenogermanium Polyoxometalate on H22 bearing C57 mice. The result of NK CPPotoxicity showed that when effect cells compared with target cells was 25:1, 60 mg/kg and 120mg/kg groups were more than control group and CPP group (p<0.05). When effect cells compared with target cells was 50:1 and 100:1, 60 mg/kg group was more than control g...
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