| The therapy of the deep partial thickness dermal burn injuring is difficult on clinic treatment, so people have been exploring the new method to make the function of derma recover better. Bone marrow MSCs have obvious plasiticity and can differentiate into the cell types in injuring organ and tissue. Therefore, the therapy of a large scale dermal burning was discussed in order that a new therapy method was obtained.In this experiment, bone marrow MSCs were obtained from the male guinea pig, and cultured, passaged. Then MSCs were transplanted into the female guinea pig of the deep partial thickness dermal burn injuring model. On a guinea pig, the left is therapy group, and the right is control groups. 3 days later after burn model was prepared, MSCs (A. group: 2×106 cell /ml; B groups: 2×107 cell /ml) were dropped on the burn injuring derma on the left group and PRMI 1640 culture medium were dropped on the right groups.4 hour later, the culture supernatant of MSCs and PRMI 1640 culture medium were dropped on the therapy groups and control groups, respectively for 30 min, which was continued for 3 days, one time a day. Then the behavior of guinea pig and the wound healing were observed .Y chromosome on the wound was detected using PCR in order to observe the survival of MSCs of male guinea pig, on the wound of female one. The results showed that MSCs can promote the healing of wound and the effect in therapy groups is better than that in control groups. Microvessel density of wound on the therapy groups (30.2800) is significantly more than that in the control groups (15.0600,P<0.05) on 15 days, and the therapy groups (34.2600) is also significantly more than that in control groups (19.3200,P<0.01) on the 30 days in group A ; that on in group B ,on 15 days the microvascular density in therapy groups (16.8200) is less than the control groups (27.0000,P<0.01) and on 30 days microvascular density on the therapy groups (28.6000) in group A is also less than that on the control groups (16.3600).The microvessel density of group A isn't significant from that of group B. The wound healing area on the therapy groups (10.0520) is significantly smaller than that in control groups (15.9600,P<0.05) on 15 days, and the wound healing area on the therapy groups (4.0640) is significantly smaller than that in control groups (5.2940,P<0.05) on the 30 days in group A; The wound healing area on the therapy groups (10.346) is significantly smaller than that in control groups (14.7240,P<0.01) on 15 days and the wound healing area on the therapy groups (4.0640) is significantly smaller than that in control groups (8.2200,P<0.01 ) on the 30 days in group B.HE stain showed, on 15 days ,newly epidermal covered the wound in all groups, on therapy groups cells differentiated better than control groups, dermal and epidermal structure clearly, cell structure of every layers epidermal is distincted and integrated, on control groups there are many died cells in epidermal and congestion in dermal. On 30 days, newly grown every layers of epidermal is well differentiated of wound in therapy groups, have newly born capillary and uncongestion. Masson stain showed, collagen is disorder and thicker, while on the 30 days, collagen is regular. Scar formation isn't obvious on the healing wound, and the healing derma isn't significantly different from the surrounding normal dermal .In all groups, hair was grown on the wound. Results of PCR is showed that MSCs can survive, reside, migrate and differentiate, and have no obvious reject-reaction on the wound of female guinea pig.In short, above results showed MSCs can promote the healing of burning wound, and the effect is good, and MSCs can play on important role during process of burn wound is MSCs differentiate into dermal stem cells healing, one of the possible mechanism. The possible mechanism is that MSCs can play a important and differentiate into dermal stem cells.
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