The Expression Of Vascular Endothelial Growth Factor Receptor1 And Progesterone Receptor In Patient With Uterine Leiomyoma

Uterine Leiomyoma (UL) is a common disease in female, Which usually lead to myomohysterectomy. However, its mechanism is not completely clear. According to some studies, progesterone (P) play a critical role in the pathogenesis of Uterine leiomyoma and some growth factors, growth factor-receptor also participate the pathogenesis of the tumor, such as epidermal growth factor (EGF), epidermal growth factor-receptor (EGF-R), IGF-1, IGF-2, vascular endothelial growth factor (VEGF), VEGF-R and so on. Among these growth factors VEGF and VEGF-R play major role in vascular growth. To study the mechanism and effect of PR, VEGF-R in the occurrence and development of UL and provide the basis of theory for instructing UL clinical treatment, we conduct the following experiment. Adopt immuno-histochemistic SP method, observe the expression of PR and VEGF-R1 to investigate the relationship between VEGF-R1, PR and UL. Patients with UL (n=40) were recruited from the inpatients at the first clinical hospital, jilin university from November 2003 to January 2004. Before operation the patients were diagnosed according to standard and had corticosteroid free for at least 3 months. The pathological diagnosis also support the diagnosis. For comparisons, sample were their myometrium (control). To detect the expression of PR and VEGFR1, first antibody is PR monoclonal antibody and VEGFR1 polyclonal antibody, respectively. Positive immunostaining is yellow-brown granulation. The VEGFR1 positive granulation peculiarly lie in cells membrane around. The PR positive granulation peculiarly lie in cells nuclear. With hemi-quantitative method to handle the results combing staining intensity and brand. To score staining intensity according to following standards. Negative is 0 score, weak but obviously stronger than the negative comparison is 1 score, clear is 2 score; strong is 3 score. the number of positive cell is 0, 0 score, 1%-25% is 1 score, 25%-50% is 2 score, ≥50% is 3 score. Adding above two scores is the expression intensity score. Results from optical microscope and electron microscope showed that VEGFR1 was detected in endothelial cell, vascular leiomyoma cell, especially abundant in the vascular endothelial cell, and it was significantly different between the UL and norm control groups. The PR was detected in the cell nuclear and there is obviously different between UL groups and the control groups. In addition, there was a close positive correlation between VEGFR1 expression and PR expression. Result from previous studies, we may presume that it is possible for VEGFR and PR were involved in the pathogenesis of UL. The VEGFR may play an important role in uterine leiomyoma enlargement; progesterone may through progesterone-receptor affect the expression of VEGF-R in leiomyoma.