Study On Glioma Glucose Metabolism Relative Genes In The Gene Expression Profile Associated With Malignant Progression Of Glioma

[ Objective ] To establish the gene expression profile associated with the malignant progression of glioma. Detecting the glucose metabolism-related genes expression patterns of human glioma specimens for helping us to study the molecular mechanisms of malignant progression of glioma.[ Methods ] 1. Collecting primary tumor specimens, tumor specimens of two recurrence and normal brain tissue of an individual patient. The classifications of tumors are ganglioglioma (WHO II), anaplastic astrocytoma (WHO III), and glioblastoma (WHO IV), respectively. (2) Detecting the gene expression patterns of above four tissues by cDNA microarray, and establishing the gene expression profile associated with malignant progression of glioma. (3) Analyzing the glucose metabolism-related genes based on the results of cDNA microarray and information from GeneBank. (4) Detecting the glucose metabolism-related genes expression patterns of different grade glioma tissues with RT-PCR for verifying the chip results.[Results] 1. 8 up-regulated and 2 down-regulated glucose metabolism relatedgenes were picked out from the gene expression profiles associated with malignant progression of glioma by bioinformatics methods;2.The glucose metabolism related genes ,such as PFK, PyK, Tra, citrate synthase, isocitrate dehydrogenase, LDH-A, L-xylulose reductase, Glut5 were proved up-regulated, and HK1 , LDH-B were proved down-regulated in gliomas by RT-PCR. [ Conclusion ] Established gene expression profile associated with malignantprogression of glioma will be important for us to clarify the molecular mechanisms of the development of glioma. The glucose metabolism-related genes Were involved in malignant progression of glioma, which may provide us new strategies and targets for glioma diagnosis and treatment.