| I. Modeling of rheumatoid arthritisObjective: To study the feasibility of the experimental method for the model of rabbit rheumatoid arthritis induced by ovalbumin. Methods: After sensitized, 8 arthritic rabbit models were induced by intra-articular injection of 5mg ovalbumin of each knee. To measure the articular diameter and the temperature changes in the articular surface skin. To randomly kill four rabbits respectively after one week and four weeks of inducement. Take out the articular synovium and cartilage for histologic examination. Results: After inducement, the articular diameters for both knees, and the temperature of the articular skin have showed an obvious increase (P<0.01). A great extent of lymphocytic infiltration, hyperemia and edema is seen inside the model synovium after one week's inducement, and it shows more marked synoviocyte hyperplasia after four weeks of inducement. Conclusion: The pathological process for the model of rabbit rheumatoid arthritis induced by ovalbumin is similar to that of human beings. The inducement method is easy and convenient, stable in effect and good for long-term maintenance. It can be a experimental model for the radiation synovectomy.II. The level of impact on the arthritic model by different dosages of Colloidal Chromic Phosphate 32PObjective: To study the impact on synovium and chondrocyte after injecting different dosages of colloidal chromic phosphate 32P into the knee joint cavities of the rabbit model of rheumatoid arthritis. Methods: To randomly divide 24 rabbits into 5 groups. Three rabbits in Group A shall serve as normal comparisons, three in Group B shall serve as model comparisons, and six rabbits respectively in Groups C, D and E. After one week of model inducement, to inject 0.5ml colloidal chromic phosphate 32Pinto the right knee joint cavities of three groups of models respectively in the dosage of 2.775MBq, 1101MBq and 44.4 MBq; and to inject the same volume of normal saline into the left knee joint cavities. Then measure the articular diameters and the changes in the temperature of articular skin. These rabbits shall be completely killed after two months. To open the joint cavities of both knees for gross and histologic examinations, and conduct corresponding scoring. Results: Rabbits in Group A do not show any changes in the articular diameters and skin temperatures, so all the scores are '0'. Rabbits in Group B show a significant increase (P<0.05) in the articular diameters and skin temperatures, which reflects increased synovia in the joint cavities and thickened synovium. Under the microscope, synoviocyte hyperplasia, formation of lymphfollicle and partial cartilage erosion can be observed. For Groups C, D and E, after injection of radionuclide, they all show an transient aggregated joint swelling, increased articular skin temperature, and different relief times and curative effect durations. For Group C, there is a distinct difference (P<0.05) between the right and left knees only regarding the treatment of synovia. For Groups D and E, all the criteria scores have maintained distinct differences (P<0.05) until execution. By comparing the right and left knees, the right knee joint only shows a slight proliferation of synoviocyte, a small amount of lymphocytic infiltration, and no obvious damage to the chondrocyte. Conclusion: There has been significant curative effects based on the radionuclide dosage calculated per synovium size. Too small dosages have a short length of curative effects, while the dosage increase can lead to longer inflammation reactions caused by the radionuclide itself. However, there have not been evidences of damage to chondrocyte. To conclude, radioisotope synovectomy has a broad range of curative safe dosage. III. The distribution of Colloidal Chromic Phosphate 32P in the main body tissues and organs of rabbit after injection into the articular cavity Objective: To study the rules of changes in the level of radioactive activity of the main issues and organs at different times after injecting colloidal chro... |
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