The Role Of TNFα And NO On Acute Necrotizing Pancreatitis Associated With Lung Injury

Aims: The objective of the study is to explore the mechanism of acute necrotizing pancreatitis(ANP) associated with acute lung injury(ALI) through making experimental models in rabbits, and to research the role of tumor necrosis factor alpha(TNF ) and nitric oxide (NO) in these models, and to provide scientific basis for treating ANP with ALI in human beings.Methods: 18 rabbits were selected in this design.They are randmized into three groups: control group(group C), treatment group(group T), experiment group(groupE). After having been anesthetized successfully,The animals of group E were induced by injecting distilled water containing 25mg of 5% Taurocholic acid into the ligated duodenal loop ,while those of group C by injecting 0.9 % sodium chloride as control ,but in group T, Octriotide has been used 1 hour before injecting 5%Taurocholic acid. Then analysis of arterial blood gas, sreum amylase, NO and TNFa were performed at 0 h , 2 h , 4 h and 6 h respectively.After 6 hours, two of animals in all groups were sacriticed, bronchoalveolar lavage was introduced in their lobes of right lung.The bronchoalveolar lavage fluid (BALF) was centrfugalized and counted neutrophil,and polymorphnuclear leukocyte(PMN) and macrophage(MA), analyzed the albumin contain. Meanwhile wet lung weight /dry lung weight ratio of their left lung was counted.In the end, the surface of lung , pancreas and histological appearence of left lung and pancreas in the microscope and electromicroscope were observed.Results: the arterial blood gas analysis, serum amylase, TNFa and NO were markedly higher at the sixth hour than those at zero hour in the group E(6.69 1.21 vs 10.65 1.67 kPa; P<0.05)(612.69 89.60 vs 151.48 11.45 somigyi ; P<0.01)(28.43 6.12vs 2.54 0.84 ; P<0.05)(83.81 6.34vsl5.31 1.32mol/L,P<0.05).There were significant changes at the sixthhour among group E and group T and C. However there were not obviously increased at sixth hour than that at zero hour in group T and group C. At sixth hour, the arterial blood gas, serum amylase, NO and TNFa were not obviously different between group T and group C. The neutrophil counts, albumin contain in BALF, and wet lung weight/dry lung weight were significantly higher in group E than those in groups T and C.By microscopic and electro-microscopic observation of the histological appearance and the supermicrostructure of cells of lung and pancreas resulted in the diagnostic standard of the acute necrotizing pancreatitis with acute lung injury in group E, but there were less changes in group T and in group C.Conclusions: This is a Successful method of making model of experimental acute negrotizing pancreatitis associated with acute lung injury; TNFa and NO were very important in inducing experimental acute necrotizing pancreatitis associated with the acute lung injury; Octreotide can significantly improve experimental acute necrotizing pancreatitis, associated with acute lung injury...