The Effect Of Glutamine On The Liver Injury By LPS

Objective: Glutamine is traditionally considered to be a "nonessential amino acid". However, recent foundational and clinical studies have provided evidences that glutamine may become "conditionally essential amino acid".For liver injury induced by LPS is related to oxidative stress and activity of kupffer cells, We explore "The effect of glutamine on the redox state of kupffer cells" to deduce "The effect of glutamine on the liver injury by LPS".Methods: Male mice were divided into three groups:Control group,LPS group,Glutamine group(Pretreatment with 3.5% glutamine solution by intraperitoneal before mice were treated with LPS plus D-galactosamine). Blood removed from the vena was analyzed for ALT,liver tissue were analyzed for the density of MDA,GSH,TNF. We induced "the effect of glutamine on the liver injury by LPS" as evidenced by ALT,MDA,GSH,TNF,morphological changes and kupffer cells phagocytosis carbon。Results:The ALT activity,the density of MDA,TNF in Glutamine group are significantly lower than LPS group, whereas the density of GSH are higher than LPS group, indicated that glutamine can regulate the redox state, reduce liver injury. Other results indicated that glutamine can enhance phagocytosis of Kupffer cells.Conclusion: Glutamine can act as an essential respiratory fuel of Kupffer cells in stress condition,enhance its phagocytosis,reduce the density of LPS in blood；Glutamine can maintain the level of GSH in liver tissue,regulate the redox state,modulate the activity of kupffer cells；Glutamine can influence cell signaling in liver,reduce liver injury induced by LPS.