Hepatic Stimulator Substance Protects Against Chemotherapeutic Liver Damage In Mice

Objective To study the protective effect of hepatic stimulator substance (HSS) on chemotherapeutic liver damage induced by cyclophosphamide and its possible mechanism. Methods 1.HSS was purified from human fetal livers. The purification steps included ultrasonication, alcohol precipitation, et al. The growth effects of HSS on SMMC-7721 cells were measured by [3H]-TdR incorporation method. 2.The LDH activities, GSH and MDA contents of hepatocytes were measured.3.The serum biochemical parameters, oxidative parameters and anti-oxidative parameters of liver tissue of male Kunming mice inoculated with S180 cells were measured. The pathology of the liver was observed with microscope. The weight of S180 sarcom was weighted. Results 1.HSS significantly promoted the incorporation of [3H]-TdR into DNA of SMMC-7721cells.2.The elevation of MDA content of hepatocytes and LDH level in supernatant of hepatocytes induced by CP were restored remarkably by HSS.3.The elevation of MDA content of liver tissue and the less of GSH content and CAT,GSH-PX,GST,SOD activities induced by CP were restored remarkably by HSS. The ALT, LDH activities of serum in HSS group which was compared with control group and CP group were not statistically distinctive. In the pathologic examination, spotty and focal necroses of hepatocytes in the liver of CP group were found with a great deal of inflammatory cells infiltration in the necrotic and portal areas, while the damage in the liver of HSS group was ameliorated obviously; The weight of S180 sarcom in CP group and HSS group was not statistically distinctive. Conclusion 1.HSS purified by this method can effectively stimulate the growth of hepatocytes.2.The effect of HSS on CP-induced hepatocyte damage maybe due to anti-oxidative activity.3.The effect of HSS on CP-induced chemotherapeutic liver damage maybe due to anti-oxidative activity.