Effect Of Tumor Necrosis Factor-α On Amino Acid Transmitter Of Hippocampus In Seizures Rats

Objectives: Tumor necrosis factor-α is an important immunity medium. It attachs itself to the occurrence and development of epilepsy. We had aimed to explore the effect of tumor necrosis factor-α on epileptic latency, degree, duration and glutamate acid-ergic and γ-aminobutyric acid-ergic neuron of hippocampus in seizures rats induced by PTZ and reveal the possible mechanism of tumor necrosis factor-α in seizure.Methods: Twenty-four adults wistar rats(180-230g) were randomly divided into three groups, NC group, PTZ group and TNF group. A dose of PTZ(35mg/kg/day) was intraperitoneally injected into the rats of PTZ group and TNF group until the kindling criterion was reached. The rats of NC group were administered with the same dose of normal saline. After kindling, the rats of TNF group were administered intraperitoneally with TNF-α (5 u g/kg),after 2 hours, the above-mentioned same dose of PTZ was administered intraperitoneally and induced seizure; the rats of PTZ group were administered intraperitoneally with the same dose of normal saline, after 2 hours, the above-mentioned same dose of PTZ was administered intraperitoneally and induced seizure. The rats were killed after seizure terminating, and perfused, fixed, drawed the materials, sectioned. To study the influence of TNF-α on glutamate acid-ergic and Y -αminobutyric acid-ergic neuron immunoreactivity by using immunohistochemistry method.Results: 1.After administering TNF-α .the epileptic latency of TNF group was shorter than PTZ group; the epileptic duration of TNF group was longer than PTZ group; the epileptic degree of TNF group was more reinforced than PTZ group(P<0.05).2. The number and optical density of glutamate acid-ergic neuron in hippocampus: the PTZ group rats was higher than that of NC group rats (P<0.01); the TNF group rats was higher than that of PTZ group rats(P<0.01) and that of NC group rats (P<0.01).3. The number and optical density of GABA-ergic neuron in hippocampus:the PTZ group rats was lower than that of NC group rats(P<0.01); The TNF group rats was lower than that of PTZ group rats(P<0.01) and that of NC group rats(P<0.01).Conclusion: 1. TNF-α reinforced the epileptic degree and shorted the epileptic latency, elongated the epileptic duration: It could obviously accelerate the occurrence and development of epilepsy.2.TNF-α had obviously reinforced the activity and excitability of glutamate acid-ergic neuron in hippocampus and suppressed the activity and excitability of GABA-ergic neuron in hippocampus. The development and episode of epilepsy were reinforced.