Periodontal Regeneration With Collagen Membrane And Bioactive Glass In Dogs

Objective The purpose of this study was to evaluate regeneration of alveolar bone and periodontal attachment, and biomaterials reaction following surgical implantation of collagen membrane associated with bioactive glass in a discriminating on-lay defect model, and to test a candidate regenerative therapy prior to clinical application. Methods Routine supra-alveolar periodontal defects were created at the premolar teeth in 3 beagle dogs, ligature wires were placed around dental cervix and were removed after 3 months. Collagen membrane associated with or without bioactive glass was grafted into the regions of premolars of beagle dogs. The regenerated tissue at 6w, 12w and 24w was evaluated through clinical examination, X-ray inspection, histological observation, respectively. The regenerated tissue at 24w was examined by X-ray energy diffractive spectrometry.Results 1. Clinical data Guided tissue regeneration with collagen membrane associated with bioactive glass resulted in statistically significant (P<0.05) reductions in probing pocket depth (2.88?.35mm), level of clinical attachment (1.88?.64mm) when compared with collagen membrane(4.75?.71mm and 3.38?.52mm) or bioactive glass (4.78?.67mm and 3.11?.60mm) at 12w . A statistically significant (P<0.01) reduction of probing pocket depth (2.00?.63mm) and level of clinical attachment (2.09?.83mm) was observed in the sites receiving collagen membrane associated with bioactive glass compared with the sites receiving collagen membrane (3.33?.71mm and 2.44?.88mm) or bioactive glass (4.11?.05mm and 3.11?.78mm) at 24w. Gingival recession values were significantly different(P<0.05) in the sites receiving collagen membrane associated with bioactive glass3(0.75?.46mm) compared with the sites receiving bioactive glass (1.67?.87mm) at 12w. 2. Descriptive analysis of radiography Bone height of regenerated alveolar was significantly increased in the sites receiving collagen membrane associated with bioactive glass. 3. Descriptive analysis of histology Newly formed alveolar bone approached and incorporated into bioactive glass in the sites receiving bioactive glass with or without collagen membrane. Residual collagen membrane could not be observed at 6w post-surgery. Bone height of regenerated alveolar was significantly increased in the sites receiving collagen membrane associated with bioactive glass compared with controls at 6w, 12w and 24w. Large amount of cementum regeneration was observed for collagen membrane combined with bioactive glass sites, whereas, which was limited for collagen membrane control sites. 4. X-ray energy diffractive spectrometry There was no statistically significant difference between groups of materials combined and normal bone for Ca/P ratio.Conclusions Bioactive glass significantly enhances alveolar bone regeneration and soft tissue healing when combined with collagen membrane.