| Objection: 1.established appropriate animal model of liver cancer and injected 32P glass microspheres (32P -GMS) by percutaneous intra-tumor, explored different doses 32P-GMS to induce the range of damage and biological effect in different time; 2.applied several methods of pathologic inspection, understood the different degree of radioactive damage to tumor with 32P-GMS, evaluated the value of MRI in diagnosis and follow-up of liver cancer; 3.based on the characteristic of damage to tumor with 32P-GMS by different ways, explored transform of the model of interventional therapy liver cancer.Material and method: We used 36 Zealand rabbits and Vx2 to establish the animal model of liver tumor. Randomized at six groups, A group without any therapy was control group; B group that injected 0.1ml lipiodol by intra-tumor was the control group of lipiodol; C, D, E and F that injected the suspension of 37MBq, 74MBq, 112MBq and 148MBq32P-GMS mixed with 0.1ml lipiodol by intra-tumor respectively was the experiment groups. The experimental animals in each group were put to death in 7, 14 and 21 days after injected 32P-GMS. The tumor tissue of injected spot was gained and observed the charges of pathologic structure with microscope and electron microscope to know clearly that radioactive internal radiation induced the damage degree of tumor cells. Meanwhile, immunohistochemistry stain was also applied to observed the charges of tumor cells and vascular. Before and after injected 32P-GMS, liver tumors were examined by MRI plain and enhanced scan.Results: l.the charges of tumor volume. A, B groups were observed that the tumor volume always increased in every week after injected 32P-GMS, the positive correlation was found between the charge of tumor volume and time; C, D groups were observed that the tumor volume decreased and the rate of restrained tumor gradually increased, however, tumor tissue still was observed in three weeks after injected 32P-GMS; 5 tumors in E group disappeared and 1 tiny tumor was observed in two weeks after injected 32P-GMS, remnant vanished after 1 week; 2 tumors in F group disappeared in lweek after injected 32P-GMS and all remaining tumors disappeared in 2 weeks after injected 32P-GMS. 2.macroscopic observation: the volume of tumors in A, B group was full and large, lots of tumor nodes and vascular were observed on the surface of tumor, the tumor tissue located the margin of cut liked fish meat, there were a mass of necrosis in the central of tumor; tumor volume, tumor nodes and vascular in C, D, E and F group were gradually diminished and even disappeared in every week after injected 32P-GMS, different degree of white fiber connective tissues were found in the lesion of tumor. 3.microscopic observation: multiple patch infiltrated cancer cells were found in tumors of A, B group, the border between tumor and normal liver parenchyma was not clear, a lot of inflammation cells infiltrated in interstitial of tumor; the lesion of tumor in C group was still found remaining cancer cell in 3 weeks after injected 32P-GMS, a few of fiber connective tissues rounded lesion, blood capillaries were observed and inflammation cells infiltrated; in D group, only few tumor tissues located the margin of lesion were found in 3 weeks after injected 32P-GMS, a lot of fiber connective tissues compassed tumor lesion; we didn't find remaining cancer cells in E group in 3 weeks after injected 32P-GMS, fiber connective tissues compassed tumor lesion completely and formed clear border between lesion and normal liver parenchyma, blood capillaries were observed hardly, the part of fiber connective tissues were hyaline degeneration and calcification, few inflammation cells infiltrated; cancer cells were not found in F group in 3 weeks after injected 32P-GMS too, however, the border between liver tumor and normal liver parenchyma was not well-define, multiple calcification patch formed and inflammation cells infiltrated obviously in the margin of tumor lesion. 4.electron microscopic observation: tumor cells in A, Bgroup were... |
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