The Experimental Study On Safety And Effectiveness Of Vaccines Administrated Simultaneously

In the light of requirement of health services for the troops, animal experiments on simultaneous administration of vaccines were carried out to study whether there are immuno-interactions and enhanced side-effects, eventually to develop protocols for emergent incubation, BALB/c mice were used in the study. Optimum dosages of the domestically produced vaccines against 4 common infectious diseases including dysentery, cholera, leptospira and haemorrhagic fever with renal syndrome(HFRS) and 2 biological warfare agents including anthrax and plague were determined for the experiments. Then the experimental studies of multiple vaccines incubated at the same time were performed to evaluate the safety and the effectiveness of different incubation schedule.The results showed that various dosage of vaccine against dysentery, cholera, leptospira and HFRS are effective enough to induce immunological reactions and 1/10,1/5,1/5 and 1/20 of human dosage of above vaccines were the most safe and effective. Local edema was observed as the main side effects after the injection of anthrax vaccine, and 1/20 of human dosage was the optimal dosage. Plague vaccine could cause local edema and death, 1/20 dosage was comparatively safe and effective.Combined incubation of vaccines against cholera, dysentery, leptospirosis and HFRS caused temporary losse of body weight and increase of WBC counts without liver and kidney damage.Low immunological reaction of dysentery vaccine was induced by this administration which had no influence to other vaccines. There were temporary increases of side-effects in multiple-vaccine groups when compared with single-vaccine groups. When the plague and the anthrax vaccine were added to the four-vaccine administration, the body weight losses and the increases of WBC counts became more obvious with abnormal liver function. The latter administration caused the low immunological reactions to anthrax, dysentery and HFRS vaccine without influences on plague, cholera and leptospirosis vaccine immunoreactions. The best multiple-vaccine administration schedules can be selected by integrating the safety and effectiveness evaluation results and these schedules need further field trial to be confirmed.