Study On Human Rare Blood Group Antigen Modification By Methoxy Polyethylene Glycol

In this study, rare blood group antigens on red blood cells(RBCs) were camouflaged by methoxy polyethylene glycol (mPEG). mPEG-BTC was chosen from three kinds of mPEG derivatives and the better condition was selected through contrast text. Based on the study, the influence of mPEG modification on cell structure and function was observed, including osmotic fragility, hemolysis, AchE, cholesterol, ATP, 2,3-DPG, deformability and morphology. Modified-RBC and unmodified-RBC were separated and the modification degree was evaluated by aqueous 2-phase system according to the partitioning characteristics alteration after PEGlyation . The storage life of mPEG modified-RBC in MAP and the modification stability during storage were also observed. The results showed that the antigenic activity of Jka Jkb k and P1 on RBCs surface disappeared after modification by mPEG-BTC at the concentration of 1.0mmol/L. The modification of mPEG has no harmful effect on RBCs morphology, structure and function, even after 21 days storage. The modification was stable during the storage period of 28 days. In vivo study showed that the survival of modified mouse RBC was normal living in recipient mouse and the half life was 14 days. In this study, we have succeeded in modifying donor RBC to transfuse those patients whose blood groups were difficult to match. Blood group matching is the prerequisite before transfusion. In clinical practice, the agglutination reaction often happens between red blood cells from the donor and the serum from the recipient even though their ABO blood systems are totally identical, especially for those patients who have received repeated blood transfusion and for those with rare blood group antigens. It is very difficult to find suitable donor blood to transfuse for those patients. In this study, we collected 29 cases of clinical difficult blood matching. The coagulation of patients serum and donor RBCs disappeared after mPEG-BTC camouflage by polybrene and antihuman globulin tests. It demonstrated that the modification on RBC with mPEG-BTC could resolve the problem of clinical difficulty in blood matching.The mPEG-BTC could camouflage the rare blood group antigens on RBC surface efficiently and had no influence on structure and function of modified RBCs. It will provide a useful strategy for preparation of universal donor blood.