| Chronic skin ulcer is a kind of popular disease, including vascular ulcer, pressure ulcer, radioactive ulcer and infectious ulcer, etc. The incidence of the disease has increased considerably in the past few years, but the cure rate was not satisfactoried. Traditional medicine treating the disease has individual advantage. The research has studied the basic mechanism of Chitosan Chinese-medicine multiple membrane treating chronic skin ulcer on the molecular level.This experiment study has researched the anti-bacteria activity of staphylococcus aureus , colibacillus , Pseudomonas aeruginosa , Klebsiella pneumoniae, Bacillus proteus, staphylococcus epidermidis of Chitosan Chinese-medicine multiple membrane by test-tube method. Back ulcer models were established on rats with literature. Then model rats were divided into three groups and treated by Chitosan Chinese-medicine multiple membrane, Physiology and Beifuji respectively. Adopted the tissues of ulcer surface separately and observe their growth circumstance by light microscope and electric microscope . Observe the variation of Epidermal growth factor , Platelet derived growth factor before and after therapy by double-anti-ELISA. The result showed that Chitosan Chinese-medicine multiple membrane has a certain of antibacterial action . The best antibacterial action in Bacillus proteus and the second is Pseudomonas aeruginosa. After model made successfully , Epidermal growth factor dropped while Platelet derived growth factor increased. The result suggested that Chitosan Chinese-medicine multiple membrane group changes obviously, reach the climax before the due date. These were different obviously compared with the other two groups (P<0.01) .The research shows Chitosan Chinese-medicine multiple membrane has great antibacterial action. It can cure the chronic skin ulcer by increasing the growth of EOF and PDGF, reach the climax before the due date. |
PDF Full Text Request