| Metabolic Syndrome was caused by genetic and environmental factors.It is composed of diabetes mellitus, visceral fat obesity, insulin resistance, hypertension, high triglycerides, and low HDL-cholesterol and other abnormalities.The relationships among these elements, especially uric acid with other elements have not been entirely understood. To explore their interactions and mechanisms, we observed relationships of the part of metabolism syndrome elements by cell culture technique and epidemiologic analysis.1. Experiment One: the effects of glucose, ox-LDL, insulin and uric acid on the proliferations and nitric oxide productions of the endothelial cell.Objective: To explore the effects of glucose, ox-LDL, insulin and uric acid on the proliferations of the endo thelial cell(ECV304) and its nitric oxide productions.Methods: The ECV304 cell was cultured in DMEM which contains different concentrations of glucose, ox-LDL, in sulin and uric acid respectively. MTT was measured to evaluate their viability with the absorbance at 490nm. Nit ric oxide concentration was determined via spectrophotom etry.Results: (1). The OD values of the endothelial cell cultured in different concentrations of glucose (5mM, 10 mM, 20 mM, 33 mM) measured by MTT were 0. 66 +0. 05, 0. 86 +0. 57, 0.89 + 0.23 and 0.81+0.15 respectively; the viability ratios in glucose concentration at 22mM were 34.8%, 30.5% and 72. 5% at 24h,48h and 72h. Nitric oxide productions of the cells cultured for 24h in different glucose concentrations were 7. 9 + 0. 3uM, 19. 0 + 3. OuM, 6.9 +0. 9uM, 2.4 +0. 5uM.(2). When ECV304 was cultured in medium containing different concentrations of insulin (0 M, 10-9M, 10-8 M, 10-7M) for 24h, the OD values evaluating the viability of the ECV304 were 0.66+0.05, 0.77 + 0.12, 0. 86 + 0. 14 and 0. 93 + 0. 17. The viability ratios were 30. 3%, 21.9%, 23. 5% at 24h, 48h and 72h when the cell was cultured in the medium with insulin at 10-8M Nitric oxide productions were 7. 9 +1.1uM, 13. 2 + 1. 8uM, 10. 6 + 0. 6uM, 5. 8 +0. 9uM in different insulin concentrations for 24h. These mean that both glucose and insulin have two-way effects on the release of the nutric oxide by ECV304.(3). OD values were 0.80 + 0. 05, 0.73 + 0.15, 0.66 + 0.01, 0.19 + 0.03 and 0.17 + 0.04 when the cell was cultured in different ox-LDL concentrations (0mg/L, 6. 25mg/L, 12. 5mg/L, 25mg/L and 50mg/L) for 72h; While the inhibition rates were 8%, 22. 9% and 78. 8% at 24h, 48h, 72h respectively when the concentration of ox-LDL was 50mg/L. Nitric oxide productions were 28. 9+1.2uM, 27.2 +2. 2uM, 15 +4uM, 6.06 + 1.2uM for 24h under different OX-LDL concentrations (0mg/L, 12.5mg/L, 25 mg/L and 50mg/L) . It showed that ox-LDL inhibited cell growth and the release of nutric oxide in a dose-dependent manner.(4). Uric acid can stimulate the proliferation of ECV304. MTT values were 1.72+0.02, 1.81 +0.03, 1. 83+0. 16 and 1. 88 +0.08 with uric acid at 0 mM, 0. 1mM, 0.2 mM, 0.4mM for 24h. Viability ratios were 9.3%, 23.3% and 4.7% with 0. 4mM uric acid at 24h, 48h and 72h. Nutric oxide releasings were 6.86+1.41uM, 12.5 + 2. 7uM, 18. 9 + 1. 8uM, 21. 1+1.4uM at 24h when uric acid in different concentrations. It indicated that uric acid may promote the cell growth and NO release.(5).MDA productions by administration of ox-LDL or LDL were 7.821 + 1.063 nmol/ml and 3.718+1.26 nmol/ml. In different uric acid concentrations (0 mM, 0.1 mM, 0.2 mM, 0. 4mM) , MDA productions were 2. 556 + 9. 8X 10-2nmol/ml, 2.889 + 8. 6X10-2 nmol/ml, 2. 778 + 9.9X10-2 nmol/ml, 2. 444 + 2. 9X 10-2nmol/ml. It means some concentrations uric acid has antioxidant properties.Conclusions of experiment one:(l). Optimal concentrat ions of glucose or insulin can promote the cell prolifer ations and nutric oxide release, while excessive glucose or insulin may have detrimental effect on the cell.(2). Ox-LDL may be detrimental to ECV304.(3). On the contrary, Uric acid may has protective effect on ECV304.2. Experiment Two: The evaluations of some elements of metabolic syndrome.Objectives:...
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