Vaccination With HB Vaccine In HBV Transgenic Mice During Pre-pregnancy And Pregnancy

Hepatitis B virus (HBV) intrauterine infection was a main route leading to mother-infant transmission, and was one reason on a large amount of HBV carriers in population. Some researches showed that HBsAg concentration in maternal blood was positive correlation with intrauterine infection rate. That is to say, the higher HBV concentration in maternal blood was, the more possible intrauterine infection occurred. So decrease of HBsAg concentration in maternal blood was important to decrease of intrauterine infection occurrence. In this study, we analyzed the variation of HBsAg concentration in maternal blood before and after injection of HB vaccine, and the effects on safety of pregnant mice and their offspring with the injection of vaccine. According to the results, the possibility of vaccination treatment was discussed, which provided experiment data for anti-HBV treatment during pregnancy.METHODS: (1)fourteen HBV transgenic(Tg) mice were randomized into A and B group with 7 mice in each group, and twelve normal BALB/C mice were also randomized into C and D group with 6 mice in each one. Group B, C and D served as controls. (2) Group A and C were injected into abdominal cavity with recombinant Hepatitis B vaccine (rHBv) containing 1.0u g of HBsAg at the beginning of 3rd wk before pregnancy and at pregnancy respectively, and group B and D were injected with l.OmL of physiological saline at the same time. (3)About 0. 3mL blood were collected from each mice in 4 groups before the start of the experiment, and at the end of 3 rd wk after the timely injection, then centrifuged, extracted blood serum and stored it. Blood sera of offspring observed for 20 days were also collected and stored. (4)Recorded the intake of water and food every two days, and their weight everyday in later pregnancy, then compared difference in all. (5)By the means of solid RIA, we detected HBsAg in sera and got their corresponding values. HBsAg concentration was computed in Microsoft Excel software and their alterations were analyzed by repeated measures analysis. (6)HBV DNA in sera of group A and B before and after injection was detected by HBV DNA quantitive analysis and was compared by t test. (7)Sera from group A, C and their offspring were detected by anti-HBs diagnosis box and the results were analyzed to show difference by fisher exact test. (8)Parts of pregnant mice and their living fetuses in each group were anatomized to do examinations on toxic, then compared between trials and controls; growth and development of newborns were observed and the index were recorded including body weight, date of ear-born, eye-open, etc. According to the above index,we identified the drug toxicity. (9)By HE stain, damage or abnormity were detected in tissues or cells of liver, kidney and spleen of each anatomized pregnant mice. RESULTS: (1)Each of mice were weighted at the beginning of the experiment, and the average weight of group A, B, C and D was 25. 16+1.35g, 25.87 + 1.21g, 24. 12 + 2. 29g and 24. 68+1. 27g, respectively. The results showed no difference among these four groups (one-way factor ANOVA, P>0.05). In addition, average HBsAg concentration in sera of group A and B before injection, which was 130. 76 + 37. 89ng/mL and 143. 07+83. 30 ng/mL, was compared, and no difference was found (t test, P>0.05). ㏒era collected three times from group A. The average HBsAg concentration was 130.76+37.89ng/mL, 148. 93+41.58ng/mL and 159. 36+79. 15ng/mL. For group B, it was 143. 07 +83. 30ng/mL, 195. 92 +114. 24ng/mL and 168.57 + 112.22ng/mL. Repeated measures analysis was performed in SPSS, and the result showed no difference between these two groups. Parts of newborns were HBsAg negative, but there was no difference between newborns of two groups. (3) HBV DNA quantitive analysis showed that HBV DNA in sera of group A and B did not decrease before and after injection. (4)The results of ELISA showed no difference of anti-HBs positive rate in group A and C at 3rd week and 6th week after vaccination, and anti-HBs negative in infants. (5) Eac...