| Background and aimAccording to the development of society and the rise of people's living standard, Cardio-cerebrovascular diseases' incidence rate which based on atherosclerosis were rising year by year. These diseases have done harm to humans' health severely. Those pathogenesis were mainly related with lipid peroxidation, endothelium injuries, immigration and proliferation of VSMC, immune adhesiveness. The traditional Chinese medicine has good function anti AS, but it's mechanisms were still not very clear. The past investigation shows that QDTMT could decrease blood glutinous degree, anti platelet aggregation, ameliorate myocardium's blood and oxygen supply.But the prevention and cure function on atherosclerosis of QDTMT have not been investigated.This experiment establish the AS model of rat by high cholesterol diet and VitD3. To observe the prevention and curefunction of QDTMT and the effects of QDTMT on the mRNA expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelium nitric oxide synthase (eNOS) of experimental atherosclerotic rat. Methodsl.The establishment of animal model 72 healthy male SD rats were randomly divided into 6 groups, 12 rats per group: model group, control group, simvastatin group, QDTMT low dosage group (QDTMTL), QDTMT middle dosage group (QDTMTM), QDTMT high dosage group(QDTMTH). The model of atherosclerosis (AS) was established by feeding high cholesterol diet and vitamin D3. This process was taken 14 weeks.2.Tissue sample collection and treatment Open the chest under the sterile condition, put rat to death by withdrawal from heart. Extract the peripheral blood mononucleus cell with the lymphocyte separation medium; Extract the aorta, thoracic aorta, abdominal aorta, some of them to keep to light microscope and electron microscope sample, other of them preserved at -70 for RT-PCR.3. Morphology observe of artery wall The fresh samples were observed by naked eye. The sample treated observed by light microscope and electron microscope.4. Half-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect ICAM-1, VCAM-1, eNOS mRNA expression in artery vessel and in the peripheral blood mononucleus cell. Analysis the changes of each group's mRNA expression of ICAM-1,VCAM-1 and eNOS .Naphthylamide Heavy nitrogen spectrophotography was adopted to detect thecontent of nitric oxide in the blood serum. To try to find out the mechanism of QDTMT prevent and cure function on AS. Results1 .Naked eye observe shows that the artery of model group rat were more sclerosis than control group. The artery of model group rat were increased thickness, changed hard. On the wall inner the artery the mass of AS were formatted. In each medication group, the sclerosis of artery were differently decreased.2.Observed though light microscope shows that the artery inner wall of model group rat were not smooth, the arrangement VSMC of middle layer were distubanced. Electron microscopeshows that the artery wall of model group rat had the endothelial cell abscission, degeneration, necrosis.And elastic fibrous layer were thickening, synthesis form VSMC increased more, some of them immigrated into infra endothelium. In each medication group, the changes of artery inner wall were differently decreased.3. The ICAM-1 and VCAM-1 mRNA expression level in the model group was significantly higher than that of control group(P<0.01) . while compared with model group, The ICAM-1 and VCAM-1 mRNA expression level of each Medication group were significantly lower (P<0.01). Furthermore, the function of QDTMTH was significantly better than QDTMTL group(p<0.05).4. The content of nitric oxide in the blood serum of model and each Medication group was significantly higher than that of control group (/*P<0.01 ) .The eNOS mRNA expression level in the model group was significantly lower than that of control group(P<0.01). While compared with model group, The eNOS mRNA expression le... |
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