Experimental Study On Effect Of Gal-HSA Magnetic Adriamycin Nanoparticles On Hepatic Carcinoma In Vitro

Objective To study the antitumor activity of Gal-HSA magnetic adriamycin nanoparticles(Gal-MADM) on liver cancer cell line HepG2 and to investigate the effect of Gal-HSA magnetic adriamycin nanoparticles on invasive ability of liver cancer cell line HepG2. Methods The effect of Gal-HSA magnetic adriamycin nanoparticles were observed by means of optical microscope electron microscope and DNA agarose gel electrophoresis. The cytotoxicity assay on HepG2 on vitro was performed with MTT method and compared with adriamycin(ADM) group human serum albumin magnetic adriamycin nanoparticle combined magnetic field (MADM-NP+M) group galactosed human serum albumin adriamycin nanoparticles(Gal-ADM-NP)group group. Cultured cells were divided into 5 groups: adriamycin(ADM) group human serum albumin magnetic adriamycin nanoparticle combined magnetic field (MADM-NP+M) group galactosed human serum albumin adriamycin nanoparticles(Gal-ADM-NP)group group galactosed human serum albumin magnetic adriamycin nanoparticle combined magnetic field (Gal-MADM-NP+M) group and Control group. After application for 24h cultured cells were harvested. The expression of Cathepsin B-mRNA were detected by the semi-quantitative reverse transcription polymerase chain reaction method(RT-PCR) and invasive ability was measured by the number of cells to penetrate polocarbonatescoated with matrigel.Results The results showed that significant changes of the cells' shapes. The inhibitory rate(IR) and 50% inhibitory concentration(IC5o) of the A groups were similar to those of the B group(p>0.05), but the C group had the obviously higher IR and IC50. Gal-MADM-NP could down-regulate the expression of of Cathepsin B-mRNA (P<0.01) . The number of cells that penetrated polycarbonates was significantly lower in group than other group (p<0.01) Conclusion The results ofthis experiment showed that the proliferation and invasive ability of hepatocellular carcinoma(HCC) cells cultured in vitro was inhibited by all groups. The cytotoxicity and inhibition to the invasive ability of Gal-MADM on the malignant hepatic tumor cells was more effective than all other groups were observed which was probably associated with the specific receptor-mediated endocytosis of hepatic galactose receptor on hepatocellular carcinoma(HCC) cells and the combination of external magnetic fields.