Effects Of QING-NING TANG On The Expression Of ET-1 And INOS MRNA In Rats With Experimental Hepatic Fibrosis

Objective To investigate the mRNA expression of the endothelin-land inducible nitric oxide synthase and assess effects of QING-NING TANG in rats with experimental liver fibrosis by carbon tetrachloride.Methods The 168 SD rats were randomly divided into two groups: the normal group and the model group. There were 18 rats in the normal group and 150 in the model group.The model were induced by injecting 40% CCU 3 ml/kg body weight and subscutaneously twice one week, at the first time the rats was given 40% CCl4 6ml/kg body weight, total for 9 weeks.The normal control group was injected normal saline as the above-mentioned dosages. After model being built, the model group was divided into the hepatiofibrosis model, the big, the middle, the small dosage QING-NING and the colchicine groups. Experiment disposal: Rats in the QING-NING groups were given QING-NING at the dosage of 22g/kg/d, 11g/kg/d and 5.5g/kg/d, rats in the colchicine group were given colchicines at the dosage of 0.5mg/kg/d, rats in the normal and the hepatiofibrosis model group were given the same capacity saline, once a day for nine weeks. All the rats were killed at the end of the eighteenth week. The severity of inflammation and fibrosis in all liver tissuses were measured by semiquantitative scoring. The expression of ET-1 mRNA and iNOSmRNA in all liver tissues was detected by in situ hybridization method.Results (1)Compared with the normal group, the severity of inflammation scoring and liver fibrosis scoring in rats of the hepatiofibrosis model group were obviously higher(P<0.01). (2)Comparedwith the hepatiofibrosis model group, the severity of inflammation scoring and the liver fibrosis scoring inrats of all the QING-NING groups and the colchicine group were obviously lower (P<0.05,P<0.01), and the severity of inflammation scoring in rats of the big dosage QING-NING group was markedly lowered(P<0.01). (3)Compared with the big dosage QING-NING group, the severity of inflammation scoring in rats of the middle dosage QING-NING group and the colchicine group were higher (P<0.01),the severity of inflammation scoring in rats of the small dosage QING-NING group was also higher(P<0.05). (4)Compared with the normal group, the expression of ET-1 mRNA and iNOS mRNA in the hepatiofibrosis model group were significantly higher(P<0.01). (5)Compared with the hepatiofibrosis model group, the expression of iNOSmRNA in all QING-NING groups were lower(P<0.05).Conlusions (1)QING-NING TANG can relieve the degree of inflammation and fibrosis of liver in rats with experimental hepatic fibrosis by carbon tetrachloride. (2)the expression of ET-1 mRNA and iNOSmRNA in the hepatiofibrosis model group were significantly higher, which suggest that ET-1 and iNOS are involved in the process of liver fibrosis. (3)QING-NING TANG can inhibit the synthesis of iNOS of liver in rats with experimental hepatic fibrosis by carbontetrachloride, which maybe is a mechanism of QING-NING TANG's antihepatiofibrosis.