Experimental Study On Vessel Cryopreservation

Objective:To study the value of mixed animo acids and rapid freezing in vessel cryopreservation, and simplify the proceeding schedule of vessel cryopreservation technique,reduce costs of preservation,promote popularization and development of that technique in clinical use.Method:120 rabbit vessels were randomly divided into three groups:the vessel of control group in cryopreservative medium containing calf serum were subjeted to rate-controled slow freezing; Those of experiment group I in cryopreservative medium containing mixed animo acids were frozen by the same method as in control group; And those of experiment group II in the same Cryopreserative medium as in control group were subjected to rapid freezing. At different preservation periods, the measurement of oxygen consumption volume and NBT tintation times, the examination of histopatholoy and ultrastructural morphology and patency rate of cryopreserved allograft arteries were carried out. Result:In each time phase,there was no significant difference between vessels in control group and expriment 1, both groups showing satisfactory effect in the measurement of oxygen consumption volume and NBT tintation times and examination of histopatholoy and ultrastructural morphology and patency rate of cryopreserved allograft arteries . But in experiment II, the obtained result of measurement and examination is not better than control group。Conclusion: Mixed animo acids can be safely applyed to vessel cryopreservation instead of calf serum 。The effect of the groups of the rapid freezing method is not better than the groups of the rate-controled slow freezing .