Studies On Anti-hepatic Fibrosis Effect Of The Active Components Of Astragali Complanali And Its Mechanism

Objective: To study active parts and quanlity control of Astragalicomplanali (AC) in protective effects of liver damage and to further investigate the effect of anti-hepatic fibrosis of active part and its mechanism.Methods: The active parts of protective liver were selected by the activity tract method with phytochemistry and pharmacology. The part C (or FAC ) were isolated and their structures were determined by spectroscopic methods . The liver fibrosis model in rat was established by using DMN. The effects of FAC on anti- peroxidation of lipid , the regulation of cytokine and the influences on collagen synthesis and degradation were observed by ELISA, RT-PCR and immunohistochemistry.Results:The extracts of AC were separated into four parts (A,B,C and D). A compound separated from part C of AC extracts was identified as CPN. The experimental results in vivo and in vitro showed that hepatic injury was inhibited by part C and part D of AC extracts significantly. The experimental results in vitro showed the hepatic injury was inhibited and the number of cell viability was improved by CPN apparently. The formation of liver fibrosis in DMN rats was prevented by FAC significantly. The MDA content was decreased, the activity of SOD was elevated in hepatic tissue significantly, the protein expression of γ- IFN and MMP-1 mRNA expression were increased and the protein expression of TIMP-1 was decreased obviously. Conclusion: Both part C and part D of AC extracts are the active parts ofprotective liver effect and CPN is one of the active components. FAC can inhibit apparently liver fibrosis in rat induced by DMN. The results of studies lay the foundation for studing and preperating one of modern Chinese medicine in treating liver fibrosis with safety and efficincy.