Case-control Study Of The Polymorphisms Of The CYP1A1 And GSTM1 Genes And Susceptibility To Esophageal Carcinoma

Objective The study intended to explore the relationship of the genetic polymorphisms of CYP1A1 and GSTM1 and susceptibility to esophageal carcinoma(EC), and the gene-gene or gene-environmental interaction. Combined genetic factors with environment factors for analysis, it could contributed to finding the cause of carcinoma, and the new way of the chemical prevention and gene engineering of carcinoma.Methods There were 89 EC patients and 98 controls in the case-control study, matched according to age, sex and living area. Cases were correctly diagnosed by histological pathology and endoscopy, and did not have any else tumor. They had not accepted any chemotherapy and radiotherapy. All cases were collected in the ChangZhi HePing hospital and the ChangZhi HeJi hospital. Controls, coming from the same hospitals at the stag, had not any tumor. Genotyping of CYP1A1 and GSTM1 were performed using PCR and PCR-RELP analysis. Combined genetic factors with environmental factors and made analysis of the univariate and multivariate and combined effect.Result Only Mspl mutation genotype showed significant difference between cases and controls, ORB=1.93(CI:1.01-3.84),ORc=3.62(95%CI:1.61-8.14). There were no significant different in the frequency distribution of three genotypes of Ile-Val polymorphisms and GSTM null genotype between EC patients and controls. Combined effects of the MspI mutation genotype and GSTM1 mull genotype indicated a notable interaction for increasing the risk of EC, OR=3.57(95%CI: 1.36-9.41). No interaction was found between the mutation genotype of Ile-Val polymorphism and GSTM1 null genotype. Multiple logistic regression showed that MspI mutation genotype was one of the most important genetic risk factors of EC. Family history of EC was another significant risk factors in genetic. Taking in fresh vegetable and fruit and eggs were protective factors of EC. In addition, the analytical results of combined effects showed that the effects of taking in fresh vegetable and fruit and eggs and MspI mutation genotype was resistant(s<1), The combined effects between family history of EC and MspI mutation genotype were synergistic( s>1).Conclusion It was suggested that Mspl polymorphisms was susceptible to EC, Family history of EC was another significant risk factor. Combined effects of the Mspl mutation genotype with GSTM1 null genotype were synergistic. Subjects with either Mspl mutation genotype or family history of EC were the higher risk population of EC, and more attentions should be paid to in the carcinoma control program.