The Contrast Between Local And General Perfusion Adenosine To The Rabbit Myocardium Ische-reperfused

Objective: The purpose of this study is to assess the myoprotective effects of local adenosine perfusion via coronary circumflex branch on ischemia-perfusion injury rat hearts model and distinGuish the effects between local perfusion and General perfusion.Method: There were three anesthetized Groups in this study named Group A(n=10, control), Group B(n=10, directly local cardial perfusion of adenosine via circumflex branch ) and Group C(n=10, General cardial perfusion of adenosine via riGht carotid artery). Ischemic arrest was induced by liGations of the coronary branch with 6/0 prolene in 30 minutes in every Group. In Group A, liGation was undone and the blood perfusion resumed. In Group B, A 1-mm diameter cannula was inserted into the coronary circumflex branch, distal to the site of the HGation, 10μ mol/L blood adenosine was perfused. In Group C, the HGation was undone and the same dosaGe adenosine perfused via riGht carotid artery. The period of time of adenosine perfusion is approximately 30 minutes in bothGroup B and C. Observed indexes include: myocardial contractility, ECG, myocardial CK-MB in blood, MPO in myocardial tissue homoGenate and myocardial ultra structure observation from electron microscope.Results: Local and General cardial perfusion Groups improved myocardial contractility and relaxation (dp/dtmax) as compared with control Group durinG reperrusion period(p<0.05). So was the MPO activity in myocardial tissue homoGenate. CK-MB leakaGe was much remarkably reduced in both Group B and C than control Group(p<0.05), but there was no siGnificant difference between Group B and C. The result is same in myocardial water content. Myocardial electron microscope observation showed that myocardial ultra structure of Group B , C was better than that of Group A.Conclusions: these experiments suggest that both local and General perfusion of adenosine have cardio-protective effects and attenuate ischemia-reperfusion injury and necrosis distinctly. There is not significantly difference between the two adenosine perfusion Groups in the mass, but local perfusion has some superiority than General perfusion in myocardial contractility, relaxation functions and MPO activity, which is beyond our expection.