The Effect Of FK506 On Expression Of Bcl-2mRNA BaxmRNA And Hepatocyte Apoptosis In Hepatic Preservation And Reperfusion

Objective To investigate Bcl-2 mRNA, BaxmRNA expression and hepatocyte apoptosis in hepatic preservation and reperfusion and effect of FK506.Methods The model of the preservation reperfusion of isolated rat liver was established. Liver tissue Bcl-2 mRNA , BaxmRNA expression and hepatocyte apoptosis were studied respectively with reverse transcription polymerase chain reaction (RT-PCR) and in situ terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) Technique.Results In FK506-treated group levels of Bcl-2 mRNA expression was obviously increased (P<0.05). BaxmRNA expression was significantly decreased (P<0.05). Hepatocyte apoptosis index was remarkably lowered (P<0.05), compared correspendingly with control group after preservation for 16, 24 and 32h followed by the same reperfusion time.Conclusion FK506 can increase Bcl-2 mRNA expression ,decrease BaxmRNA expression and inhibit hepatocyte apoptosis. FK506 may paly a positive effect in preventing hepatic preservation reperfusion injury.