| Objective The purpose of this experiment was to establish the rat modelof subarachnoid hemorrhage ( SAH ) by injecting autologous blood into cisterna magna and to investigate the expression of the insulin-like growth factor-1 (IGF-1) in the cerebral cortex , thalamus and hippocampus within 5 days after subarachnoid hemorrhage.Methods 60 adult Wistar rats were randomly divided into groups of normal, sham operation and SAH. 0. 3ml autologous blood was obtained from the cut-off tail of rat and subarachnoid hemorrhage was induced by injection of autologous blood into the cisterna magna. In the sham operation group, 0. 3ml saline instead of autologous blood was injected into the cisterna magna of rat , and in normal group the animals were normal rats. The SAH group and the sham operation group were divided to 7 groups by different postoperative time spot (lh, 2h, 8h, Id, 2d, 3d and 5d after operation). The expression of IGF-1 were measured by in situ hybridization in the brain tissues of rat.Results In both normal group and sham operation group, only few cells with expression of IGF-1 appeared, showing no significant difference(t=0~ 2.00, P>0. 05). In SAH group, The expression of IGF-1 in cerebral cortex began to raise at lh, peaked at 24h and decreased to normal at 5d after SAH; the expression in thalamus and hippocampus began to raise at 2h, peaked at 48h and decreased to normal at 5d after SAH. The peak time in cerebral cortex was 24h earlier than that in thalamus and hippocampus.Conclusion In SAH group, the expression of IGF-1 is more intensive than that in control groups, the IGF-1 expression of cerebral cortex appears earlier than that in thalamus and hippocampus. It Auggests that IGF-1 may play a important role in the pathophysiology of SAH.
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