| Fetal Bacteria Granuloma after Truma is a new kind of lethal disease with a quick progress. Before 21st century very few dermatologist knew the special disease. It was found and named by the department of dermatology in XIJING hospital in 1996.The result of Transmission electron microscope suggest that there are bacteria similar to Bacillus in the macrophage cells which were taken from the lesion on the skin of the patients. Many times of strict bacteria culture have suggested that the pathogen of FBGT was a kind of anaerobic short Bacillus. The bacteria was regarded as Propionibacterium acnes or Actinomycete according to the evaluation from chemistry examination, but it was proved to be Propionibacterium acnes by API system. We saved apatient according to the result of the medicine sensitivity test.1. Molecular Biology Identification the Pathogen of FetalBacteria Granuloma after TrumaIt is commonly regarded that P. acnes is a kind of bacteria that normally living on the skin with a weak power to cause a disease, but in FBGT, it can cause all the patients die. Here comes a question that is: is the bacteria cultured from the skin of the FBGT patients be P. acnes surely? The API system suggests that it was P. acnes, but according to the evaluation from chemistry examination, whether it is a kind of Actinomycete or P. acnes are not determined. So we should study the bacteria more and declear the right Genus or Species of the special pathogen.In the current microbiology, the polyphasic taxonomy is regarded as the best way to distinguish bacteria'41, it has many informations such as gene etc, among them, 16S rRNA sequence analysis includes information of evolution, and is suitable for every kinds of taxonomy, while DNA-DNA hybridization can measureate the hybridization rate, which serve as a guarantee in the exact taxonomy. Both may be used to identificate unknown bacteria.We mensurated the 16S rRNA sequence of the pathogen, and analysied it in the genebank with Blast, the result of 16S rRNA sequencing Analysis fits the sequence in the Genbank very well, with a rate odf 99. 9%. So we can say thatthe pathogen cultured from the skin of FBGT patients is P. acnes.In order to make our result accurate, we asked the microbiology lab in the Chinese science academy to help us in the DNA-DNA hybridization of the pathogen and the type strain of P. acnes. The rate of hybridization >70%, so the two strains of bacteria is belong to the same Species.We approved that the pathogen of FBGT is P. acnes.2. Primary building the animal model of Fetal Bacteria Granuloraa after TrumaFBGT is a kind of infective disease, in order to study the etiology and find effective ways to treat the patients, animal model must be built.Koch's postulates is regarded as the main discipline in deciding whether an animal model for an infective disease is successed or not. That is, pathogen must survive in the body of the experiment animal, not in the healthy ones and can be cultured out, then inject the pathogen into healthy animals can cause the same signs and symptoms, another culture must be done in the injected animals and must get the same pathogen as injected.According to the result of pre-experiment, we select the white-house rabbit to carry on our study.We used the following methods: First, prednisone was used to suppress the immunity of the experiment animal, the bacteria got through pure cultivation was made intosuspension and injected into the derma of experiment animal, then there comes lesions in the injected sites, we cut down the lesions and send it to histopathology and transmission electron microscope examination, we also cultured the bacteria from the injected site again, and send it to 16S rRNA gene sequencing Analysis and API system evaluation. Few tested animals had some nervous system symptoms, we sent them for MRI examination, the same process like skins lesions were used to their brains.In our experiment, all the injected site comes up with erythema , the erythema with a lower bacteria concertration di...
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