| DNA is the carrier of genetic information and plays an especially important role in the course of genetic. Investigation of DNA is important in the life sciences field. DNA is the main target molecule that exists in anticancer and antiviral therapies. All cell and virus carry DNA. Normal heredity function of DNA depends on the physical and chemical interaction of other molecules with DNA.Interaction of DNA with small molecules, especially drug molecules affected physiological and physical chemical character of DNA and changed the transfer and copy of DNA. Hence it is meaning that interactions of small molecules with DNA are investigated to understand bind of DNA with protein, anticancer mechanism and to design and synthesize new drug. In medicament, it possible to foresee major steps forward in our understanding of the molecular basis of disease, both from attack by external pathogens and internally from variations within the human genome resulting in a plethora of new molecular therapeutic targets for drug design and discovery.A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding drugs, such as the gel mobility shift assay, H NMR, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry. However, most of these methods are indirect and discontinuous, and require various labeling strategies. Moreover, the turnover time and cost of the conventional assays place a limit on the applicability of large-scale screening of drug candidates. In recent years, there has been a growing interest in the electrochemical investigations of interactions between anticancer drugs and other DNA-targeted molecules and DNA, because electrochemical ones provide rapid, simple and low-cost point-of-care detection of specific drugs.Based on the above view, in this paper we first provides a general introduction and conclusion of the research about the interaction of drug with DNA; secondly, the mechanisms studies of the bioflavonoids and metal complexes interaction with DNA was investigated. quercetin-SAM-modified gold electrode surface is an effective mediator for electrocatalytic oxidation of DA.Part I General Introduction and ConclusionIn this part, the development in the electrochemical studies of DNA, including electrochemical behavior of DNA, electrochemical analysis of DNA, interaction of DNA with other matter and DNA modified electrode. It is important to study DNA interaction with drug in theory and in practice. Reports about the action of bioflavonoids and metal complexes with DNA are very few in the word. And we study action of bioflavonoids and metal complexes with DNA at DNA modified electrode and in solution by mainly electrochemical methods.Part II Electrochemical Behavior of Quercetin and Interaction of Quercetin with DNAQuercetin is one of Bioflavonoids, which are a large family of organic compounds widely distributed in the plant kingdom. In fact, quercetin have been reported a widely pharmacological properties such as antiviral, anti-allergic, anti-platelet, anti-inflammatory and anti-tumor activities, and possibly even protective effects against chronic diseases. Recently, the considerable interest in a modifier layer of DNA immobilized on the electrode surface is due to use identify most important matter (including drugs and potential pollutants), the recognizing specific base sequences of DNA and the elucidation of the mechanism of drugs action. The studies value is that found rapid and sensitive electrochemical methods, and discusses action mechanism of other molecules with DNA. The specialty about DNA modified electrode is simple, rapid and low-cost detection of DNA.The interaction of quercetin with DNA was investigated by surface electrochemical methods, DNA modified electrode. The result is that quercetin can bind with DNA at DNA modified glass carbon electrode. The action mode is electrostatic attaract. An intrinsic binding constant, K of (3.80+0.3)x104 M-1, and n of 1 were obtained.Part...
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