| AFE is the most serious obstetric complication that incidence is low and death rate is very high but can not be anticipated and it can develop to MODS in a short time. Because AFE is very urgent, it is difficult to investigate the patho-genesis, prevention, diagnosis and treatment. MPO exists in neutrophil(PMN) as a indicator which reflects the degree of PMN infiltration sensitively, when PMN is stimulated. NF - kB is an ubiquitous rapid - response transcription factor binding to target genes encoding proteins involved cytokines, adhesion molecules and chemokines, when the cells are activated and NF - kB translocates to the nucleus. It may be relevant to the occurrence and development of MODS. Dex has anti - inflammatory and anti - allergic function and can alleviate the inflammatory progress.The purpose of this experiment is to create the entrance of amniotic fluid model to investigate the expression of NF - kB and MPO in the pulmonary of ratsand the inhibition of Dex.Material and MethodsMaterial1. Animals: Adult fertile Wistar rats ( Be pregnant on 20 days) weighting 320 - 380g (the Experimental Animals Breeding Department of China Medical University). Select the rats which is satisfying to the experimental demander. Divide into five groups randomly: control group, amniotic fluid group, therapic group A( amniotic fluid + Dex) , meconium group and therapic group B( meconi-um + Dex).2. Agent and equipment: Rabbit - anti - rat NF - kB polyclone antibody, MPO reaction kits. Blood pressure monitor (Siemens) , Supercentrifuge ( Ameri-can type DU PONT-SORVALL SUPER-T21 ) , Electrophoresis apparatus( BIO - RAD POWERPAC 300) ,Homogenizer( Heidolph) , Figure image analysis system. Method;1. Great amniotic fluid and 1% meconium fluid.2. Great the entrance of amniotic fluid model.3. Isolation of nuclear proteins.4. Westen - blot analysis of the expression of NF - KB p65: The results were expressed as relative optical density by scanning densitometer of a Bio -Image analysis system.5. Measurement of MPO in pulmonary tissue: MPO activity (u/g) = A A x (13.5)/tissue wet weight. A A is the change of absorptivity from 30s to 90s.6. HE stain; Observe the pathologic changes of pulmonary tissue.7. Immunohistochemistry of NF - KB p65: Semiquantitativecount method was used to count the number of p65 positive cells in 10 visual fields of every smear under 400 micro - scope.8. Statistical analysis: Data were analyzed by using SPSS statistical software and were expressed as median ?standard error. Analysis of variance and t test were used to analysize all data. Size of test was 0.05 and the value of P <0.05 was considered statistically significant.Results1. HE stain results; Dropsy,bleeding and PMN,macrophage leukomonocyte infiltration were in four experimental groups. But no process was found in control group.2. Measurments of the NF - KB and MPO activities: NF - KB and MPO activation remained basal level in control, meanwhile were significantly increased in four experimental groups. There were statistical differences in these groups (P <0.05).3. Immunohistochemical localization of NF - KB p65; Less positive cells were in control. In contrast,localization for p65 in the nucleus was in four experimental groups, and there was an evidence of significantly higher in meconiumgroup than in the others( P < 0.05 ).DiscussionMyeloperoxidase ( MPO ) exists in neutrophil ( PMN ) as an indicator which reflects the degree of PMN infiltration sensitively. In this study, dropsy, bleeding and PMN, macrophage leukomonocyte infiltration were in four experimental groups, consistent with MPO activity, especially in meconium group. These results indicate that inflammation participate AFE - induced pulmonary injury, and presume that embolus in amniotic fluid contribute chemiotaxis to PMN, then oxidant and protease derived from PMN induce injury of microvascular. The inflammatory kinin released from pulmonary alveoli macrophage leads to the micro-vascular leakage and injury of lu...
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